Serebrijski I G, Kazakova S M, Tsygankov Y D
Institute of Genetics and Selection of Industrial Microorganisms, Moscow, U.S.S.R.
FEMS Microbiol Lett. 1989 May;50(1-2):203-6. doi: 10.1016/0378-1097(89)90486-2.
The transposon-loaded plasmid pAS8-121, incapable of autonomous replication in Gram-negative bacteria of non-enteric group, was transferred to Methylobacillus flagellatum KT wild type strain MFK1. The transconjugants arose at a frequency of 10(-7) per donor cell. The majority of the transconjugants tested exhibited the transfer of all selected chromosomal markers at rather high (10(-4)-10(-6) per donor cell) but similar frequencies. Only one of the obtained donors, designated MFK 64, was capable of mobilizing M. flagellatum KT chromosome in a polarized manner. The integrated nature of the plasmid in this and other MFK1 (pAS8-121) derivatives was supported by the results of DNA-DNA hybridization.
携带转座子的质粒pAS8 - 121不能在非肠道菌群的革兰氏阴性菌中自主复制，它被转移到了鞭毛甲基杆菌KT野生型菌株MFK1中。转接合子的出现频率为每供体细胞10^(-7)。大多数测试的转接合子显示所有选定的染色体标记物的转移频率相当高（每供体细胞10^(-4)-10^(-6)）但相似。所获得的供体中只有一个，命名为MFK 64，能够以极化方式动员鞭毛甲基杆菌KT染色体。DNA - DNA杂交结果支持了该质粒在这个和其他MFK1（pAS8 - 121）衍生物中的整合性质。
