Jantzen E, Bryn K, Bovre K
Acta Pathol Microbiol Scand B. 1976 Aug;84(4):177-88. doi: 10.1111/j.1699-0463.1976.tb01923.x.
Sixty-four strains of Neisseria, Moraxella, and Acinetobacter were screened for cellular monosaccharides by gas-liquid chromatography and other chromatographic techniques. The four sugars ribose, glucose, glucosamine, and 2-keto-3-deoxyoctonate (KDO) were detected in all strains. Heptose was detected only in "true neisseriae" (Neisseria gonorrhoeae, N. meningitidis, N. sicca, N. cinerea, N. flavescens, and N. elongata) and in the tentaively named species Moraxella urethralis. Some marked interspecies dissimilarities within groups were revealed. Thus, N. ovis and M. atlantae were characterized by the presence of mannose. Intraspecies differences were also encountered. N. meningitidis strains of serogroups B and C were distinguished from strains of serogroup A by their sialic acid content. This sugar was also detected in two out of three examined strains of M. nonliquefaciens. In Acinetobacter, heterogeneity of monosaccharide patterns was rather pronounced. The results show the applicability of gas chromatographic "monosaccharide" profiles fo whole cells or extracted carbohydrate in bacterial classification and identification, including differentiation at the subspecies level. In addition, such profiles may be useful for monitoring during purification of cellular polysaccharides.
采用气液色谱法和其他色谱技术，对64株奈瑟菌属、莫拉克斯菌属和不动杆菌属细菌进行了细胞单糖筛查。在所有菌株中均检测到核糖、葡萄糖、氨基葡萄糖和2-酮-3-脱氧辛酸（KDO）这四种糖类。仅在“真奈瑟菌”（淋病奈瑟菌、脑膜炎奈瑟菌、干燥奈瑟菌、灰色奈瑟菌、微黄奈瑟菌和延长奈瑟菌）以及暂定命名的尿道莫拉克斯菌中检测到庚糖。在各菌群内部还发现了一些显著的种间差异。因此，绵羊奈瑟菌和亚特兰大莫拉克斯菌的特征是含有甘露糖。种内差异也有出现。B群和C群脑膜炎奈瑟菌菌株与A群菌株在唾液酸含量上有所不同。在三株检验的非液化莫拉克斯菌中有两株也检测到了这种糖类。在不动杆菌中，单糖模式的异质性相当明显。结果表明，气相色谱“单糖”图谱可用于细菌分类和鉴定，包括亚种水平的区分，以及全细胞或提取的碳水化合物的分类和鉴定。此外，这种图谱对于细胞多糖纯化过程中的监测可能也有用。
