用气相色谱法检测长奈瑟菌全细胞中的3-脱氧-D-甘露-2-辛酮糖酸和L-甘油-D-甘露庚糖。
Detection by gas chromatography of 3-deoxy-D-manno-2-octulosonic acid and L-glycero-D-manno-heptose in whole cells of Neisseria elongata.
作者信息
Guerrant G O, Moss C W
出版信息
J Clin Microbiol. 1986 Mar;23(3):425-30. doi: 10.1128/jcm.23.3.425-430.1986.
Abstract
Lipopolysaccharide components 3-deoxy-D-manno-2-octulosonic acid and L-glycero-D-manno-heptose were detected in hydrolysates from whole cells of Neisseria elongata by gas-liquid chromatography. Cells from a single plate were hydrolyzed, and carbohydrate components were converted to aldononitrile and O-methyloxime acetate derivatives for subsequent analyses by gas-liquid chromatography. 3-Deoxy-D-manno-2-octulosonic acid was well separated from other cell components as the O-methyloxime acetate derivative. With both derivatives, L-glycero-D-manno-heptose was readily identified by their different retention times. The procedure requires only a relatively small number of cells, and detection is accomplished without prior isolation of the lipopolysaccharide.
摘要
通过气液色谱法在长奈瑟菌全细胞水解产物中检测到脂多糖成分3-脱氧-D-甘露-2-辛酮糖酸和L-甘油-D-甘露庚糖。将来自单个平板的细胞进行水解，并将碳水化合物成分转化为醛糖腈和O-甲基肟乙酸酯衍生物，以便随后通过气液色谱法进行分析。3-脱氧-D-甘露-2-辛酮糖酸作为O-甲基肟乙酸酯衍生物与其他细胞成分得到了很好的分离。对于这两种衍生物，L-甘油-D-甘露庚糖可通过其不同的保留时间轻松鉴定。该方法仅需要相对少量的细胞，并且无需事先分离脂多糖即可完成检测。
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