通过包裹在多层脂质体中来提高重组腺相关病毒载体的转导效率。
Improvement of transduction efficiency of recombinant adeno-associated virus vector by entrapment in multilamellar liposomes.
作者信息
Mizuno M, Yoshida J
机构信息
Department of Neurosurgery, Nagoya University School of Medicine.
出版信息
Jpn J Cancer Res. 1998 Apr;89(4):352-4. doi: 10.1111/j.1349-7006.1998.tb00570.x.
Abstract
Recombinant adeno-associated virus (AAV) has attracted considerable interest as a potential vector for human gene therapy, but its transduction efficiency is quite low. The present study demonstrated AAV vector-associated liposomes to be more effective for in vitro gene transfer to human glioma cells than are liposomes containing plasmid DNA. Using vector-associated liposomes increased the transduction efficiency more than 10-fold compared to liposomes containing plasmid DNA and more than 6-fold compared to AAV alone. From these results, AAV vector-associated liposomes appear to be a good candidate for in vivo gene delivery to human gliomas.
摘要
重组腺相关病毒（AAV）作为一种潜在的人类基因治疗载体已引起了广泛关注，但其转导效率相当低。本研究表明，与含有质粒DNA的脂质体相比，AAV载体相关脂质体在体外对人胶质瘤细胞进行基因转移时更有效。与含有质粒DNA的脂质体相比，使用载体相关脂质体可使转导效率提高10倍以上，与单独使用AAV相比可提高6倍以上。基于这些结果，AAV载体相关脂质体似乎是用于人胶质瘤体内基因递送的良好候选物。
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