Delporte C, Hoque A T, Kulakusky J A, Braddon V R, Goldsmith C M, Wellner R B, Baum B J
Gene Therapy and Therapeutics Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892, USA.
Biochem Biophys Res Commun. 1998 May 29;246(3):584-8. doi: 10.1006/bbrc.1998.8668.
AdhAQP1, a recombinant adenovirus encoding the human water channel aquaporin 1 (AQP1), has been shown to be useful for gene therapy of salivary glands rendered hypofunctional following irradiation. Here we utilized AdhAQP1 to examine the relationship between AQP1 expression and fluid movement across a polarized salivary epithelial cell monolayer. In response to a 440 to 340 mosm gradient, net fluid movement across cells infected with AdhAQP1 was approximately 10-fold that seen in uninfected cells or cells infected with a control virus. At a multiplicity of infection (MOI) of 5, fluid movement was linear for 15-30 min. Increasing the osmotic gradient resulted in a proportional increase in fluid movement. At low virus MOIs (0.1-1.0), fluid movement was markedly enhanced relative to that seen at higher MOIs (approximately 5.10), where the level of AQP1 expression and number of cells transduced were considerably greater. We conclude that significant, osmotically-obliged fluid movement in a salivary cell monolayer with low basal water permeability does not require high levels of AQP1 expression.
AdhAQP1是一种编码人类水通道蛋白1（AQP1）的重组腺病毒，已被证明可用于对放疗后功能减退的唾液腺进行基因治疗。在此，我们利用AdhAQP1来研究AQP1表达与液体跨极化唾液上皮细胞单层转运之间的关系。在440至340毫渗量梯度的刺激下，AdhAQP1感染细胞的净液体转运量约为未感染细胞或感染对照病毒细胞的10倍。在感染复数（MOI）为5时，液体转运在15 - 30分钟内呈线性。增加渗透压梯度会导致液体转运量成比例增加。在低病毒MOI（0.1 - 1.0）时，相对于高MOI（约5.10）时，液体转运显著增强，而在高MOI时，AQP1表达水平和转导细胞数量要多得多。我们得出结论，在基础水通透性较低的唾液细胞单层中，显著的、受渗透压驱动的液体转运并不需要高水平的AQP1表达。
