Reischl U, Feldmann K, Naumann L, Gaugler B J, Ninet B, Hirschel B, Emler S
Institute for Medical Microbiology and Hygiene, University of Regensburg, Germany.
J Clin Microbiol. 1998 Jun;36(6):1761-4. doi: 10.1128/JCM.36.6.1761-1764.1998.
Direct sequencing of the 16S rRNA gene (16S rDNA) of Mycobacterium celatum isolates showed ambiguities, suggesting heterogeneity. Cloned 16S rDNA yielded two copies of the gene, which differed by insertion of a thymine at position 214 and by additional mismatches. Restriction fragment length polymorphism analysis confirmed the presence of two copies of 16S rDNA within the bacterial chromosome.
对塞拉分枝杆菌分离株的16S rRNA基因(16S rDNA)进行直接测序时出现了模糊性,提示存在异质性。克隆的16S rDNA产生了该基因的两个拷贝,它们在第214位插入了一个胸腺嘧啶,并且存在其他错配差异。限制性片段长度多态性分析证实细菌染色体中存在两个16S rDNA拷贝。