Lee T H, Chen Y M, Chou H N
Department of Zoology, National Taiwan University, Taipei, ROC.
Toxicon. 1998 Feb;36(2):247-55. doi: 10.1016/s0041-0101(97)00128-1.
This is the first report on microcystins from Microcystis aeruginosa Kützing in Taiwan. A total of nine strains of cyanobacteria have been isolated from eutrophic aquaculture ponds and water reservoirs. By mouse toxicity assay, six of the nine strains had LD100 in the range of 25-100 mg per kg mouse for dried bacterial mass. Microcystin-LR and -RR were found in all toxic strains and their contents ranged from 0.11-10.06 microg and 0.08 2.21 microg per mg of dried bacteria, respectively. Microcystin-RA, a minor component found only in M. TN-2 and M. CY-1 strains, was identified as a new microcystin. All three toxins were isolated by a serial separation on an LH-20 column, Si-flash column chromatography and reverse phase HPLC. Toxins were further identified by comparing their FABMS, 1H and 1H-1H COSY NMR spectra with the authentic microcystin-LR. Several other microcystin-like compounds were also found in the cultured strains and their structures are being determined.
这是台湾地区关于铜绿微囊藻微囊藻毒素的首份报告。从富营养化水产养殖池塘和水库中总共分离出九株蓝藻。通过小鼠毒性试验，九株菌株中的六株对小鼠干菌体的半数致死量（LD100）在每千克小鼠25至100毫克范围内。在所有有毒菌株中均发现了微囊藻毒素-LR和-RR，其含量分别为每毫克干菌0.11至10.06微克和0.08至2.21微克。微囊藻毒素-RA仅在M. TN-2和M. CY-1菌株中被发现，是一种次要成分，被鉴定为一种新的微囊藻毒素。通过在LH-20柱、硅胶快速柱色谱和反相高效液相色谱上进行系列分离，分离出了所有三种毒素。通过将其快原子轰击质谱（FABMS）、1H和1H-1H化学位移相关谱（COSY）核磁共振光谱与标准微囊藻毒素-LR进行比较，进一步鉴定了毒素。在培养菌株中还发现了其他几种类似微囊藻毒素的化合物，其结构正在确定中。
