Lu S, Syrjänen K, Havu V K, Syrjänen S
MediCity Research Laboratory, Faculty of Medicine, University of Turku, Finland.
Arch Dermatol Res. 1996 Dec;289(1):40-5. doi: 10.1007/s004030050150.
To assess the role of human papillomavirus (HPV) in the aetiology of extragenital Bowen's disease (EBD), a series of 91 cases were analysed using in situ hybridization (ISH) with whole genomic DNA probes of HPV types 1, 2, 4, 6, 7, 11, 12, 15, 16, 18, 26, 36, 37, 39, 42, 55 and 59. No HPV DNA was found in any of the samples tested. A polymerase chain reaction (PCR) was also used to analyse 37 of the 91 samples, using both the consensus primers MY09/MY11 which amplify a large number of HPV types from the L1 region and the degenerate primers CP65/CP70, which amplify the complete set of epidermodysplasia verruciformis (EV) HPV types. All the cases were also negative in the PCR. The results suggest that EBD is not HPV-related, at least in immunocompetent patients.
为评估人乳头瘤病毒（HPV）在生殖器外鲍温病（EBD）病因学中的作用，我们使用1、2、4、6、7、11、12、15、16、18、26、36、37、39、42、55和59型HPV的全基因组DNA探针，通过原位杂交（ISH）对91例病例进行了分析。在所检测的任何样本中均未发现HPV DNA。还使用聚合酶链反应（PCR）对91个样本中的37个进行了分析，使用了能从L1区域扩增大量HPV类型的通用引物MY09/MY11以及能扩增疣状表皮发育不良（EV）HPV完整类型的简并引物CP65/CP70。所有病例在PCR检测中也均为阴性。结果表明，至少在免疫功能正常的患者中，EBD与HPV无关。
