组蛋白与溴脱氧尿苷取代的DNA结合比与正常DNA结合更紧密。
Histones bind more tightly to bromodeoxyuridine-substituted DNA than to normal DNA.
作者信息
Lin S, Lin D, Riggs A D
出版信息
Nucleic Acids Res. 1976 Sep;3(9):2183-91. doi: 10.1093/nar/3.9.2183.
Abstract
Using a membrane filter assay, we have obtained results from both kinetic and competition experiments indicating that histones bind more strongly to bromodeoxyuridine-substituted DNA than to normal DNA. At 37 degrees C in our standard buffer of 0.2 M ionic strength, the rate of dissociation of histones H1, H2, and h4 from BrdU-substituted DNA is respectively 7, 4, and 2 times slower than it is from normal DNA. Competition experiments show an even greater difference between BrdU-substituted and normal DNA with respect to histone binding. The tighter binding of histones to BrdU-substituted DNA is of interest because of the known effects of BrdU on eukaryotic chromosome condensation and staining, virus induction, and the inhibition of differentiation.
摘要
通过膜过滤分析，我们从动力学实验和竞争实验中均获得了结果，这些结果表明组蛋白与溴脱氧尿苷取代的DNA的结合比与正常DNA的结合更强。在37摄氏度、离子强度为0.2M的标准缓冲液中，组蛋白H1、H2和H4从溴脱氧尿苷取代的DNA上解离的速率分别比从正常DNA上解离的速率慢7倍、4倍和2倍。竞争实验表明，在组蛋白结合方面，溴脱氧尿苷取代的DNA与正常DNA之间的差异更大。由于溴脱氧尿苷对真核染色体浓缩和染色、病毒诱导以及分化抑制的已知作用，组蛋白与溴脱氧尿苷取代的DNA的更紧密结合令人感兴趣。
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