通过紫外线辐射使乳糖阻遏物光化学附着于溴脱氧尿苷取代的乳糖操纵基因。
Photochemical attachment of lac repressor to bromodeoxyuridine-substituted lac operator by ultraviolet radiation.
作者信息
Lin S Y, Riggs A D
出版信息
Proc Natl Acad Sci U S A. 1974 Mar;71(3):947-51. doi: 10.1073/pnas.71.3.947.
Abstract
The transducing phage lambdah80dlac carries the lac operator, whereas wild-type lambdah80 does not. We find that in high salt (0.18 M KCl), ultraviolet radiation causes the formation of a very stable complex between repressor and 5-bromodeoxyuridine (BrdU)-substituted lambdah80dlac but not to BrdU-lambdah80 DNA. Studies with inducers of the lac operon confirm the specificity of attachment. In low slat (0.01 M KCl), ultraviolet radiation will also attach repressor nonspecifically to BrdU-lambdah80 DNA. The stability of the complex suggests that covalent bonds are formed. We also report that another regulatory protein, the catabolite gene activator protein, can be attached similarly to DNA.
摘要
转导噬菌体λh80dlac携带乳糖操纵基因，而野生型λh80则不携带。我们发现，在高盐（0.18M KCl）条件下，紫外线辐射会导致阻遏物与5-溴脱氧尿苷（BrdU）取代的λh80dlac之间形成非常稳定的复合物，但不会与BrdU-λh80 DNA形成复合物。利用乳糖操纵子诱导剂进行的研究证实了结合的特异性。在低盐（0.01M KCl）条件下，紫外线辐射也会使阻遏物非特异性地结合到BrdU-λh80 DNA上。复合物的稳定性表明形成了共价键。我们还报告了另一种调节蛋白，即分解代谢基因激活蛋白，也能以类似方式结合到DNA上。
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Photochemical attachment of lac repressor to bromodeoxyuridine-substituted lac operator by ultraviolet radiation.通过紫外线辐射使乳糖阻遏物光化学附着于溴脱氧尿苷取代的乳糖操纵基因。
Proc Natl Acad Sci U S A. 1974 Mar;71(3):947-51. doi: 10.1073/pnas.71.3.947.
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The lac repressor-operator interaction. 3. Kinetic studies.乳糖阻遏蛋白与操纵基因的相互作用。3. 动力学研究。
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