Crowell W A, Leininger J R
Am J Vet Res. 1976 Sep;37(9):1075-9.
Twelve cats were used to study autolytic changes in glomerular morphology and compare these with lesions of naturally occurring feline renal disease. The 12 cats had normal clinical, urinary, and blood features. One kidney (0-hour control) was excised immediately after a given cat was euthanatized, and portions of it were prepared for light and electron microscopy. The opposite kidney (autolytic) remained in situ for selected postmortem intervals, up to 24 hours, at which time it was similarly processed. Renal tissues from 4 additional cats (3 with proteinuria and 1 with diabetes mellitus) were processed and examined for comparison. Zero-hour control kidneys had the following mean quantitations: renal weight was 9.9 g; glomerular diameter, 83 mum; number of cells per glomerulus in 1-mum section was 63; and diameter of cell nuclei was 6.3 mum for mesangial, 6.7 mum for visceral epithelial, and 6.4 mum for endothelial. In comparison with 0-hour control kidneys, autolytic kidneys had increased weight and glomerular diameter, but the diameter of cell nuclei decreased. Basement membrane thickness and glomerular cell numbers did not differ between 0-hour control and autolytic kidneys. Kidneys from 4 diseased cats had increased glomerular diameter and glomerular basement membrane changes characterized by hyalin thickening and dense deposits. These changes are compatible with a lesion diagnosis of membranous glomerulonephritis.
选用12只猫来研究肾小球形态的自溶变化，并将这些变化与自然发生的猫肾脏疾病的病变进行比较。这12只猫的临床、尿液和血液特征均正常。在给定的猫安乐死后，立即切除一侧肾脏（0小时对照），并将其部分组织制备用于光镜和电镜检查。另一侧肾脏（自溶）在选定的死后间隔时间内原位保留，最长24小时，之后同样进行处理。另外4只猫（3只蛋白尿猫和1只糖尿病猫）的肾脏组织也进行了处理和检查以作比较。0小时对照肾脏的平均定量数据如下：肾脏重量为9.9克；肾小球直径为83微米；在1微米切片中每个肾小球的细胞数为63个；系膜细胞核直径为6.3微米，脏层上皮细胞核直径为6.7微米，内皮细胞核直径为6.4微米。与0小时对照肾脏相比，自溶肾脏的重量和肾小球直径增加，但细胞核直径减小。0小时对照肾脏和自溶肾脏之间的基底膜厚度和肾小球细胞数量没有差异。4只患病猫的肾脏肾小球直径增加，肾小球基底膜有变化，表现为透明样增厚和致密沉积物。这些变化符合膜性肾小球肾炎的病变诊断。
