Anson J J, Allen K D
Department of Microbiology, Whiston Hospital, Merseyside, England, UK.
Br J Biomed Sci. 1997 Dec;54(4):237-9.
The performance and cost-effectiveness of CHROMagar Candida in the isolation and identification of Candida species from female genital tract specimens were investigated. Eight hundred and forty-three specimens were inoculated in parallel onto malt extract agar and CHROMagar Candida. Yeasts isolated on CHROMagar were provisionally identified by morphology and pigmentation. Those isolated on malt extract agar were further identified by a germ tube test, and negative isolates were identified biochemically. A cost comparison was performed, detailing labour and material costs. Of 119 yeast isolates, seven were detected only on CHROMagar. Mixed Candida spp. from three specimens were only differentiated on CHROMagar. There was a 100% correlation between the results of the germ tube test and provisional identity on CHROMagar. CHROMagar Candida facilitated the presumptive identification of yeast isolates from the female genital tract, and the detection of mixed populations. The cost of the media was higher than malt extract agar and germ tube testing, but the rapid identification of isolates to species level and ease of use may be of sufficient advantage to justify the extra cost.
研究了科玛嘉念珠菌显色培养基（CHROMagar Candida）从女性生殖道标本中分离和鉴定念珠菌属的性能及成本效益。将843份标本同时接种于麦芽提取物琼脂和科玛嘉念珠菌显色培养基上。在科玛嘉念珠菌显色培养基上分离出的酵母通过形态和色素沉着进行初步鉴定。在麦芽提取物琼脂上分离出的酵母通过芽管试验进一步鉴定，阴性分离株进行生化鉴定。进行了成本比较，详细列出了劳动力和材料成本。在119株酵母分离株中，7株仅在科玛嘉念珠菌显色培养基上被检测到。来自3份标本的混合念珠菌属仅在科玛嘉念珠菌显色培养基上得以区分。芽管试验结果与科玛嘉念珠菌显色培养基上的初步鉴定结果之间存在100%的相关性。科玛嘉念珠菌显色培养基有助于从女性生殖道中对酵母分离株进行推测性鉴定，并检测混合菌群。该培养基的成本高于麦芽提取物琼脂和芽管试验，但能快速将分离株鉴定到种水平且使用方便，可能具有足够的优势来证明额外成本的合理性。
