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用于检测四种念珠菌的显色和荧光膜过滤法的比较。

Comparison of chromogenic and fluorogenic membrane filtration methods for detection of four Candida species.

作者信息

Bauters T G, Nelis H J

机构信息

Laboratory for Pharmaceutical Microbiology, Department of Pharmaceutical Analysis, Ghent University, Harlebekestraat 72, 9000 Ghent, Belgium.

出版信息

J Clin Microbiol. 2002 May;40(5):1838-9. doi: 10.1128/JCM.40.5.1838-1839.2002.

DOI:10.1128/JCM.40.5.1838-1839.2002
PMID:11980972
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC130677/
Abstract

CHROMagar Candida medium was inoculated with a variety of clinical samples (n = 282) by using membrane filtration instead of swabbing or streaking. This modified CHROMagar procedure increased the ability to detect yeast by 16.7%. Compared to an enzymatic two-step membrane filtration method with fluorogenic substrates, it yielded similar recovery but was slower.

摘要

使用膜过滤而非拭子或划线法，将多种临床样本（n = 282）接种于CHROMagar念珠菌培养基。这种改良的CHROMagar方法使酵母检测能力提高了16.7%。与使用荧光底物的酶促两步膜过滤法相比，其回收率相似，但速度较慢。

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本文引用的文献

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Laryngoscope. 2002 Apr;112(4):708-12. doi: 10.1097/00005537-200204000-00021.
2
Evaluation of CHROMagar Candida for rapid screening of clinical specimens for Candida species.评估科玛嘉念珠菌显色培养基用于临床标本中念珠菌属快速筛查的效果。
Mycoses. 1999 Apr;42(1-2):61-5. doi: 10.1046/j.1439-0507.1999.00406.x.
3
Membrane filtration test for rapid presumptive differentiation of four Candida species.用于四种念珠菌属快速初步鉴别的膜过滤试验
J Clin Microbiol. 1999 May;37(5):1498-502. doi: 10.1128/JCM.37.5.1498-1502.1999.
4
An evaluation of the cost-effectiveness of using CHROMagar for yeast identification in a routine microbiology laboratory.在常规微生物实验室中使用科玛嘉培养基进行酵母鉴定的成本效益评估。
J Med Microbiol. 1998 Jul;47(7):623-8. doi: 10.1099/00222615-47-7-623.
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Evaluation of CHROMagar Candida medium for the isolation and direct identification of yeast species from the female genital tract.评估科玛嘉念珠菌培养基用于从女性生殖道中分离和直接鉴定酵母菌种的效果。
Br J Biomed Sci. 1997 Dec;54(4):237-9.
6
Use of CHROMagar Candida for genital specimens in the diagnostic laboratory.在诊断实验室中，CHROMagar念珠菌培养基用于生殖器标本检测。
J Clin Pathol. 1997 Jul;50(7):563-5. doi: 10.1136/jcp.50.7.563.
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Evaluation of CHROMagar Candida medium for the isolation and presumptive identification of species of Candida of clinical importance.评估科玛嘉念珠菌显色培养基用于分离和初步鉴定具有临床重要性的念珠菌属菌种。
Diagn Microbiol Infect Dis. 1996 Apr;24(4):201-4. doi: 10.1016/0732-8893(96)00063-6.