Bouma A, De Jong M D, Kimman T G
DLO-Institute of Animal Science and Health (ID-DLO), Lelystad, The Netherlands.
Res Vet Sci. 1998 Mar-Apr;64(2):167-71. doi: 10.1016/s0034-5288(98)90014-5.
In this study, the influence of maternal immunity against pseudorabies virus (PRV) on the development of a PRV-specific T-cell response in pigs was investigated. Pigs with or without maternal immunity were challenged by inoculation with wild-type PRV, or were vaccinated with 783 and subsequently inoculated. Peripheral blood lymphocytes, collected after vaccination and/or inoculation, were used for PRV-induced lymphocyte proliferation assays in vitro as a measure of a PRV-specific T-cell response. In unvaccinated pigs, the presence of maternal immunity did not inhibit the development of a T-cell response after inoculation with PRV. In contrast, maternal immunity did inhibit the development of a PRV-induced response after intramuscular vaccination. Moreover, vaccinated pigs without maternal immunity shed virus for a shorter period after inoculation than vaccinated pigs with maternal immunity. The greater T-cell response induced by the vaccine might have contributed to the more rapid clearance of PRV in these pigs.
在本研究中，调查了母猪抗伪狂犬病病毒（PRV）免疫力对猪体内PRV特异性T细胞反应发育的影响。有或没有母源免疫力的猪通过接种野生型PRV进行攻毒，或接种783疫苗后再进行接种。在接种疫苗和/或接种后采集外周血淋巴细胞，用于体外PRV诱导的淋巴细胞增殖试验，作为PRV特异性T细胞反应的一种衡量指标。在未接种疫苗的猪中，母源免疫力的存在并不抑制接种PRV后T细胞反应的发育。相比之下，母源免疫力确实抑制了肌肉注射疫苗后PRV诱导反应的发育。此外，没有母源免疫力的接种疫苗猪在接种后病毒排毒时间比有母源免疫力的接种疫苗猪短。疫苗诱导的更强T细胞反应可能有助于这些猪中PRV的更快清除。
