Murphy A A, Santanam N, Morales A J, Parthasarathy S
Department of Gynecology and Obstetrics, Emory University, Atlanta, Georgia 30322, USA.
J Clin Endocrinol Metab. 1998 Jun;83(6):2110-3. doi: 10.1210/jcem.83.6.4823.
Inflammatory processes have been hypothesized to mediate some of the clinical sequelae associated with endometriosis. The peritoneal fluid (PF) of women with endometriosis is known to contain more inflammatory cells and their associated cytokines, chemokines, and growth factors. This work provides strong evidence for oxidative stress in the PF of women with endometriosis. 1) The low density lipoprotein (LDL) isolated from the PF of subjects with endometriosis shows a small but detectable increase in electrophoretic mobility compatible with mildly oxidized LDL compared with LDL isolated from the plasma of the same subjects and PF of controls. 2) Isolated PF-LDL of endometriosis subjects is more readily oxidized in vitro than PF-LDL of controls, or LDL isolated from plasma. 3) Vitamin E content is significantly lower in endometriosis PF compared with controls, and compared with plasma of women with endometriosis and controls. No difference is seen between plasma and PF of control subjects. 4) The ratio of phosphatidylcholine/lyso phosphatidylcholine (Ptd/lyso PtdCho) in the PF of endometriosis subjects is significantly lower compared with PF of controls. Taken together, these data provide strong evidence for a pro-oxidant environment in the peritoneal cavity of women with endometriosis. Lyso PtdCho, a product derived from phospholipase A2 action on peroxidized phosphatidylcholine and a potent chemotactic factor for monocytes and T-lymphocytes, is elevated in endometriosis. We hypothesize that the increased presence of lipid peroxidation products in the PF of endometriosis subjects may, at least partly, account for the recruitment of leukocytes, the increase in macrophage activation, the secretion of monocyte--macrophage-derived cytokines, and the endometrial growth-promoting activity associated with endometriosis.
炎症过程被认为介导了一些与子宫内膜异位症相关的临床后遗症。已知患有子宫内膜异位症的女性腹膜液（PF）中含有更多的炎症细胞及其相关的细胞因子、趋化因子和生长因子。这项研究为子宫内膜异位症患者腹膜液中的氧化应激提供了有力证据。1）从患有子宫内膜异位症的受试者的腹膜液中分离出的低密度脂蛋白（LDL），与从同一受试者的血浆和对照组的腹膜液中分离出的LDL相比，其电泳迁移率有小幅但可检测到的增加，这与轻度氧化的LDL相符。2）与对照组的腹膜液LDL或从血浆中分离出的LDL相比，子宫内膜异位症患者分离出的腹膜液LDL在体外更容易被氧化。3）与对照组相比，子宫内膜异位症患者的腹膜液中维生素E含量显著降低，与子宫内膜异位症患者和对照组的血浆相比也是如此。对照组受试者的血浆和腹膜液之间没有差异。4）与对照组的腹膜液相比，子宫内膜异位症患者的腹膜液中磷脂酰胆碱/溶血磷脂酰胆碱（Ptd/lyso PtdCho）的比例显著降低。综上所述，这些数据为患有子宫内膜异位症的女性腹膜腔中促氧化环境提供了有力证据。溶血磷脂酰胆碱是磷脂酶A2作用于过氧化磷脂酰胆碱产生的产物，是单核细胞和T淋巴细胞的有效趋化因子，在子宫内膜异位症中升高。我们假设，子宫内膜异位症患者腹膜液中脂质过氧化产物的增加可能至少部分解释了白细胞的募集、巨噬细胞活化的增加、单核细胞 - 巨噬细胞衍生细胞因子的分泌以及与子宫内膜异位症相关的促进子宫内膜生长的活性。
