Cousins D V, Skuce R A, Kazwala R R, van Embden J D
Australian Reference Laboratory for Bovine Tuberculosis, Agriculture Western Australia, South Perth, Australia.
Int J Tuberc Lung Dis. 1998 Jun;2(6):471-8.
The Tuberculosis in Animals Subsection of the International Union Against Tuberculosis and Lung Disease (IUATLD) recently identified a need to standardize the deoxyribonucleic acid (DNA) strain typing of Mycobacterium bovis. The standard method for strain typing of M. tuberculosis isolates cannot be directly extrapolated to M. bovis due to the low copy number of IS6110 identified in the majority of M. bovis strains, particularly from cattle. To improve the resolution of M. bovis strains, alternative methods and additional DNA probes have been investigated. In combination with studies of published literature, laboratories performing M. bovis DNA fingerprinting were surveyed. Results of these surveys allowed us to reach consensus and to make recommendations for DNA typing of M. bovis isolates, which hopefully will lead towards a standardized approach to the DNA fingerprinting of this organism. This approach, in conjunction with conventional epidemiological traceback approaches, should facilitate more accurate and effective investigations into the epidemiology, maintenance and transmission of M. bovis within and between man and domesticated, feral and wild animals, both at a local and a global level.
国际防痨和肺部疾病联盟（IUATLD）动物结核病小组最近确定需要对牛分枝杆菌的脱氧核糖核酸（DNA）菌株分型进行标准化。由于在大多数牛分枝杆菌菌株（尤其是来自牛的菌株）中鉴定出的IS6110拷贝数较低，结核分枝杆菌分离株菌株分型的标准方法不能直接外推至牛分枝杆菌。为提高牛分枝杆菌菌株的分辨力，已对替代方法和额外的DNA探针进行了研究。结合已发表文献的研究，对进行牛分枝杆菌DNA指纹分析的实验室进行了调查。这些调查结果使我们能够达成共识，并对牛分枝杆菌分离株的DNA分型提出建议，有望形成该生物体DNA指纹分析的标准化方法。这种方法与传统的流行病学追溯方法相结合，应有助于在地方和全球层面更准确有效地调查牛分枝杆菌在人和家养、野生及野生动物之间的流行病学、维持和传播情况。
