从意大利人类身上分离出的牛分枝杆菌临床分离株的分子分析。
Molecular analysis of clinical isolates of Mycobacterium bovis recovered from humans in Italy.
作者信息
Lari Nicoletta, Rindi Laura, Bonanni Daniela, Tortoli Enrico, Garzelli Carlo
机构信息
Dipartimento di Patologia Sperimentale, Biotecnologie Mediche, Infettivologia ed Epidemiologia, Via San Zeno, 35/39 - I-56127 Pisa, Italy.
出版信息
J Clin Microbiol. 2006 Nov;44(11):4218-21. doi: 10.1128/JCM.01216-06. Epub 2006 Sep 20.
Abstract
In order to achieve a better knowledge of Mycobacterium bovis epidemiology in Italy, 42 clinical isolates from humans were genotyped. Predominant molecular patterns were found in one cluster of 15 isolates sharing spoligotype (ST482), variable-number tandem repeat (VNTR), and IS6110-based restriction fragment length polymorphism (one 1.9-kb band) profiles and in two clusters of 6 and 3 Mycobacterium bovis BCG isolates differing by one VNTR character. The remaining 18 isolates yielded unique profiles. Our results confirm the potential utility of spoligotyping and VNTR typing as a major typing system of M. bovis isolates.
摘要
为了更好地了解意大利牛分枝杆菌的流行病学情况，对42株来自人类的临床分离株进行了基因分型。在一个由15株分离株组成的簇中发现了主要的分子模式，这些分离株具有相同的间隔寡核苷酸分型（ST482）、可变数目串联重复序列（VNTR）以及基于IS6110的限制性片段长度多态性（一条1.9 kb条带）图谱；另外在两个分别由6株和3株牛分枝杆菌卡介苗（Mycobacterium bovis BCG）分离株组成的簇中也发现了主要分子模式，这两个簇中的分离株仅在一个VNTR特征上存在差异。其余18株分离株产生了独特的图谱。我们的结果证实了间隔寡核苷酸分型和VNTR分型作为牛分枝杆菌分离株主要分型系统的潜在效用。
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