Gronow M, Thackrah T M, Lewis F A
Biochem J. 1976 Aug 1;157(2):507-9. doi: 10.1042/bj1570507.
Non-histone proteins from rat liver nuclei and chromatin were shown to be hydrolysed in 0.1M or-1M-NaOH solutions both at 4 degrees and 18 degrees C; 24h in 1M-NaOH at 18 degrees C is sufficient to break down approx. 77% of these proteins to low-molecular-weight peptides. Loss of protein material banding in the region of pH5.5-8.0 has been demonstrated by isoelectric focusing in polyacrylamide gels, and fine high-molecular-weight bands are no longer visible on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. The results indicate that care must be taken when analysing non-histone-protein fractions to avoid exposure to alkaline pH conditions.
大鼠肝细胞核和染色质中的非组蛋白在4℃和18℃下于0.1M或1M氢氧化钠溶液中均会被水解；在18℃下于1M氢氧化钠中放置24小时足以将约77%的这些蛋白质分解为低分子量肽。通过聚丙烯酰胺凝胶等电聚焦已证明在pH5.5 - 8.0区域蛋白质条带的损失，并且在十二烷基硫酸钠/聚丙烯酰胺凝胶电泳上不再可见精细的高分子量条带。结果表明，在分析非组蛋白部分时必须小心，以避免暴露于碱性pH条件。
