从猪肝中分离（铜、锌）硫蛋白。
Isolation of (copper, zinc-) thioneins from pig liver.
作者信息
Bremner I, Young B W
出版信息
Biochem J. 1976 Jun 1;155(3):631-5. doi: 10.1042/bj1550631.
Abstract
The copper-binding proteins with mol. wt. of approx. 12000 in the cytosol of pig liver have been identified as copper-thioneins. They contain variable amounts of zinc, with approx. 10% total metal in the protein. The molar cysteine/metal ratio is approx. 2:1.
摘要
猪肝脏细胞质中分子量约为12000的铜结合蛋白已被鉴定为铜硫蛋白。它们含有不同量的锌，蛋白质中总金属含量约为10%。半胱氨酸与金属的摩尔比约为2:1。
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1
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Ann N Y Acad Sci. 1964 Dec 28;121:404-27. doi: 10.1111/j.1749-6632.1964.tb14213.x.
2
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Biochem J. 1962 Dec;85(3):480-5. doi: 10.1042/bj0850480.
3
4
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5
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7
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