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从猪肝中分离（铜、锌）硫蛋白。

Isolation of (copper, zinc-) thioneins from pig liver.

作者信息

Bremner I, Young B W

出版信息

Biochem J. 1976 Jun 1;155(3):631-5. doi: 10.1042/bj1550631.

DOI:10.1042/bj1550631
PMID:949324
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1172886/
Abstract

The copper-binding proteins with mol. wt. of approx. 12000 in the cytosol of pig liver have been identified as copper-thioneins. They contain variable amounts of zinc, with approx. 10% total metal in the protein. The molar cysteine/metal ratio is approx. 2:1.

摘要

猪肝脏细胞质中分子量约为12000的铜结合蛋白已被鉴定为铜硫蛋白。它们含有不同量的锌，蛋白质中总金属含量约为10%。半胱氨酸与金属的摩尔比约为2:1。

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本文引用的文献

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DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.圆盘电泳。II. 方法及其在人血清蛋白中的应用。
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The effect of glutathione on protein sulphydryl groups in rat-liver homogenates.谷胱甘肽对大鼠肝脏匀浆中蛋白质巯基的影响。
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3
Metallothionein: a cadmium and zinc-containign protein from equine renal cortex. II. Physico-chemical properties.金属硫蛋白：一种来自马肾皮质的含镉和锌的蛋白质。II. 物理化学性质。
J Biol Chem. 1961 Sep;236:2435-42.
4
The particulate half-cystine-rich copper protein of newborn liver. Relationship to metallothionein and subcellular localization in non-mitochondrial particles possibly representing heavy lysosomes.新生肝脏富含半胱氨酸的颗粒状铜蛋白。与金属硫蛋白的关系以及在可能代表重型溶酶体的非线粒体颗粒中的亚细胞定位。
Biochem Biophys Res Commun. 1974 Feb 4;56(3):661-8. doi: 10.1016/0006-291x(74)90656-1.
5
The effect of copper loading on the distribution of copper in rat liver cytosol.
Biochem Med. 1973 Aug;8(1):78-91. doi: 10.1016/0006-2944(73)90011-2.
6
Conversion of metallothionein into Cu-thionein, the possible low molecular weight form of neonatal hepatic mitochondrocuprein.金属硫蛋白向铜硫蛋白的转化，可能是新生儿肝脏线粒体铜蛋白的低分子量形式。
FEBS Lett. 1974 Aug 30;44(3):293-7. doi: 10.1016/0014-5793(74)81161-0.
7
Hepatic copper- and zinc-binding proteins in ruminants. 2. Relationship between Cu and Zn concentrations and the occurrence of a metallothionein-like fraction.
Br J Nutr. 1974 Sep;32(2):293-300. doi: 10.1079/bjn19740082.
8
Hepatic copper-and zinc-binding proteins in ruminants. 1. Distribution of Cu and Zn among soluble proteins of livers of varying Cu and Zn content.
Br J Nutr. 1974 Sep;32(2):283-91. doi: 10.1079/bjn19740081.
9
Neonatal hepatic mitochondrocuprein. IV. Sulfitolysis of the cystine-rich crude copper protein and isolation of a peptide containing more than 35 percent half-cystine.新生儿肝脏线粒体铜蛋白。IV. 富含胱氨酸的粗铜蛋白的亚硫酸解及一种半胱氨酸含量超过35%的肽段的分离。
Biochim Biophys Acta. 1971 Jan 19;229(1):143-54.
10
The relationship between the zinc status of pigs and the occurrence of copper- and Zn-binding proteins in liver.猪的锌状态与肝脏中铜锌结合蛋白的出现之间的关系。
Br J Nutr. 1976 Mar;35(2):245-52. doi: 10.1079/bjn19760028.