Walbot V, Coe E H
Department of Biology, Washington University, St. Louis, Missouri 63130.
Proc Natl Acad Sci U S A. 1979 Jun;76(6):2760-4. doi: 10.1073/pnas.76.6.2760.
The recessive nuclear gene iojap of Zea mays conditions a permanent, heritable deficiency in the ability of the plastid to differentiate. iojap-affected plastids contain a normal genome as evidenced by comparison of the restriction endonuclease digestion patterns of affected and normal plastids. iojap-affected plastids contain neither detectable ribosomes nor high molecular weight RNA; the affected plastids do not incorporate exogenous amino acids into protein. The lesion in plastid ribosome content occurs early in organ ontogeny because iojap-mediated albino stripes can occupy entire clones within affected leaves.
玉米的隐性核基因iojap导致质体分化能力出现永久性、可遗传的缺陷。通过比较受影响质体和正常质体的限制性内切酶消化模式证明,受iojap影响的质体含有正常基因组。受iojap影响的质体既没有可检测到的核糖体,也没有高分子量RNA;受影响的质体不能将外源氨基酸掺入蛋白质中。质体核糖体含量的损伤发生在器官个体发育的早期,因为iojap介导的白化条纹可以占据受影响叶片内的整个克隆。