Research School of Biosciences, University of Kent, CT2 7NJ, Canterbury, UK.
Cytotechnology. 1996 Jan;22(1-3):17-24. doi: 10.1007/BF00353920.
The advent of new technologies for analysis of biopolymers by mass spectrometry has revolutionised strategies for recombinant protein characterization. The principal recent developments have been matrix-assisted laser desorption/ionization and electrospray ionization mass spectrometry. Using these tools, accurate molecular mass determinations can now be obtained routinely-often using minute (picomole-femtomole) quantities of protein or protein fragments. These techniques have proved indispensible for detailed characterization of the post-translational modifications of recombinant proteins produced by eukaryotic systems. Glycosylation is arguably the most important and complex of these modifications and has prompted widespread use of these new techniques. In this mini-review article I describe recent advances in the use of mass spectrometry for analysis of recombinant glycoproteins.
质谱分析生物聚合物的新技术的出现彻底改变了重组蛋白分析的策略。最近的主要进展是基质辅助激光解吸/电离和电喷雾电离质谱。使用这些工具，现在可以常规地获得准确的分子量测定值，通常只需要微量（皮摩尔-飞摩尔）的蛋白质或蛋白质片段。这些技术对于详细分析真核系统产生的重组蛋白的翻译后修饰是必不可少的。糖基化可以说是最重要和最复杂的修饰之一，这促使了这些新技术的广泛应用。在这篇迷你综述文章中，我描述了质谱在重组糖蛋白分析中的最新进展。
