Grison R, Grezes-Besset B, Schneider M, Lucante N, Olsen L, Leguay J J, Toppan A
Unité de Phytotechnologie, Sanofi Recherche, Centre de Labège, France.
Nat Biotechnol. 1996 May;14(5):643-6. doi: 10.1038/nbt0596-643.
Constitutive overexpression of a protein involved in plant defense mechanisms to disease is one of the strategies proposed to increase plant tolerance to fungal pathogens. A hybrid endochitinase gene under a constitutive promoter was introduced by Agrobacterium-mediated transformation into a winter-type oilseed rape (Brassica napus var. oleifera) inbred line. Progeny from transformed plants was challenged using three different fungal pathogens (Cylindrosporium concentricum, Phoma lingam, Sclerotinia sclerotiorum) in field trials at two different geographical locations. These plants exhibited an increased tolerance to disease as compared with the nontransgenic parental plants.
组成型过表达参与植物病害防御机制的蛋白质是提高植物对真菌病原体耐受性的策略之一。通过农杆菌介导的转化，将一个由组成型启动子驱动的杂交内切几丁质酶基因导入一个冬性油菜（甘蓝型油菜变种油用油菜）自交系。在两个不同地理位置的田间试验中，用三种不同的真菌病原体（同心柱盘孢、茎点霉、核盘菌）对转基因植株的后代进行挑战。与非转基因亲本植株相比，这些植株对病害的耐受性有所提高。
