Goh M S, Gorton T S, Forsyth M H, Troy K E, Geary S J
Department of Pathobiology, U-89, 61 N Eagleville Rd, University of Connecticut,Storrs, CT 06269-3089,USA.
Microbiology (Reading). 1998 Nov;144 ( Pt 11):2971-2978. doi: 10.1099/00221287-144-11-2971.
The identification of a gene (gapA) from Mycoplasma gallisepticum with homology to the P1 cytadherence gene of Mycoplasma pneumoniae is reported. The gapA gene is a 2895 bp ORF encoding a protein with a molecular mass of 105 kDa. Nucleotide sequence analysis of the gapA gene revealed 45% homology to the M. pneumoniae P1 gene, 46% homology to the Mycoplasma genitalium MgPa gene and 47% homology to the Mycoplasma pirum P1-like protein gene. It has a 64 mol % A+T content compared to 46, 60 and 72 mol % respectively for the P1, MgPa and the P1-like protein genes. As with the P1 and MgPa genes, gapA is a central gene in a multi-gene operon, but unlike the P1 and MgPa genes, there is only a single copy of gapA in the genome. GapA is a trypsin-sensitive surface-exposed protein. Chicken tracheal-ring inhibition-of-attachment assays, using anti-GapA Fab fragments, resulted in 64% inhibition of attachment. These results indicated that GapA plays a role in cytadherence of M. gallisepticum to host cells.
已报道从鸡毒支原体中鉴定出一个与肺炎支原体P1黏附基因具有同源性的基因（gapA）。gapA基因是一个2895 bp的开放阅读框，编码一种分子量为105 kDa的蛋白质。gapA基因的核苷酸序列分析显示，它与肺炎支原体P1基因的同源性为45%，与生殖支原体MgPa基因的同源性为46%，与梨支原体P1样蛋白基因的同源性为47%。其A+T含量为64 mol%，而P1、MgPa和P1样蛋白基因的A+T含量分别为46 mol%、60 mol%和72 mol%。与P1和MgPa基因一样，gapA是一个多基因操纵子中的核心基因，但与P1和MgPa基因不同的是，基因组中gapA只有一个拷贝。GapA是一种对胰蛋白酶敏感的表面暴露蛋白。使用抗GapA Fab片段进行的鸡气管环附着抑制试验导致64%的附着抑制。这些结果表明，GapA在鸡毒支原体对宿主细胞的黏附中起作用。
