Martelli A M, Riccio M, Bareggi R, Manfioletti G, Tabellini G, Baldini G, Narducci P, Giancotti V
Dipartimento di Morfologia Umana, Università di Trieste, Trieste, Italy.
J Histochem Cytochem. 1998 Jul;46(7):863-4. doi: 10.1177/002215549804600710.
The intranuclear distribution of HMGI/Y proteins was analyzed by immunofluorescent staining in several cell lines using a polyclonal antibody that stained a fibrogranular network. In actively growing 3T3 fibroblasts, HMGI/Y proteins were mainly localized to heterochromatin masses, whereas in quiescent cells they were more diffusely distributed. Double labeling experiments showed a co-localization of HMGI/Y with DNA topoisomerase IIalpha. These results are in agreement with previously published biochemical data and indicate a possible involvement of HMGI/Y proteins in several nuclear functions, including chromatin organization and gene expression.
使用一种能对纤维颗粒网络进行染色的多克隆抗体，通过免疫荧光染色分析了几种细胞系中HMGI/Y蛋白的核内分布。在活跃生长的3T3成纤维细胞中，HMGI/Y蛋白主要定位于异染色质团块，而在静止细胞中它们分布更为弥散。双重标记实验显示HMGI/Y与DNA拓扑异构酶IIα共定位。这些结果与先前发表的生化数据一致，并表明HMGI/Y蛋白可能参与多种核功能，包括染色质组织和基因表达。
