Grassauer A, Egorov A Y, Ferko B, Romanova I, Katinger H, Muster T
Institute of Applied Microbiology, University of Agricultural Sciences, Vienna, Austria.
J Gen Virol. 1998 Jun;79 ( Pt 6):1405-9. doi: 10.1099/0022-1317-79-6-1405.
During the 1996 influenza epidemic in Vienna we obtained influenza A virus specimens (Vienna/47/96, Vienna/81/96) which grow efficiently in African green monkey kidney (Vero) cells but not in embryonated chicken eggs. Amplification of the specimens in Vero cells resulted in progeny that agglutinated human but not chicken erythrocytes. Reassortment analysis suggested that the haemagglutinin (HA) might be responsible for the host restriction. Vero cells were infected with the Vienna/47/96 virus and then transfected with reconstituted ribonucleoprotein complexes containing HA genes from egg-adapted strains. Subsequent selective passages in embryonated chicken eggs resulted in selection of transfectant viruses, growing in eggs and containing the transfected HAs. The results demonstrate that host restriction of the Vero-adapted Vienna/47/96 virus is due to its HA. Moreover, the experiments showed that the Vienna/47/96 strain can be used as helper virus for reverse genetics experiments.
在1996年维也纳流感流行期间，我们获得了甲型流感病毒标本（维也纳/47/96、维也纳/81/96），这些标本在非洲绿猴肾（Vero）细胞中能高效生长，但在鸡胚中不能生长。在Vero细胞中扩增这些标本产生的子代能凝集人红细胞而不能凝集鸡红细胞。重配分析表明，血凝素（HA）可能是宿主限制的原因。用维也纳/47/96病毒感染Vero细胞，然后用含有来自适应鸡胚毒株HA基因的重组核糖核蛋白复合物进行转染。随后在鸡胚中进行选择性传代，筛选出了能在鸡胚中生长并含有转染HA的转染病毒。结果表明，适应Vero细胞的维也纳/47/96病毒的宿主限制是由其HA引起的。此外，实验表明，维也纳/47/96毒株可作为反向遗传学实验的辅助病毒。
