Lietman T, Brooks D, Moncada J, Schachter J, Dawson C, Dean D
F. I. Proctor Foundation, University of California San Francisco 94143-0142, USA.
Clin Infect Dis. 1998 Jun;26(6):1335-40. doi: 10.1086/516373.
We determined whether patients with chronic conjunctivitis in whom direct fluorescent antibody (DFA) tests revealed genus-specific chlamydial antigens (but not species-specific Chlamydia trachomatis antigens) were infected with Chlamydia psittaci or Chlamydia pneumoniae. Patients were divided into a case group of possible non-trachomatis chlamydial conjunctivitis and a control group of nonchlamydial conjunctivitis on the basis of examination and DFA testing. Species-specific primers were used to amplify C. trachomatis, C. psittaci, and C. pneumoniae DNA with polymerase chain reaction (PCR). Four (27%) of 15 samples from the case group were positive for C. psittaci or C. pneumoniae DNA, whereas none of 24 control samples were positive. Sequencing revealed a C. pneumoniae, an avian C. psittaci, and two mammalian C. psittaci strains. A short course of oral antibiotic treatment appears to be inadequate for patients with non-trachomatis chlamydial conjunctivitis. Ocular infections due to C. pneumoniae and C. psittaci may be more common than previously recognized and can be identified by DFA and PCR.
我们确定了直接荧光抗体（DFA）检测显示属特异性衣原体抗原（但非种特异性沙眼衣原体抗原）的慢性结膜炎患者是否感染了鹦鹉热衣原体或肺炎衣原体。根据检查和DFA检测结果，将患者分为可能的非沙眼衣原体性衣原体结膜炎病例组和非衣原体性结膜炎对照组。使用种特异性引物通过聚合酶链反应（PCR）扩增沙眼衣原体、鹦鹉热衣原体和肺炎衣原体的DNA。病例组的15份样本中有4份（27%）鹦鹉热衣原体或肺炎衣原体DNA呈阳性，而24份对照样本均无阳性。测序显示有一株肺炎衣原体、一株禽源鹦鹉热衣原体和两株哺乳动物源鹦鹉热衣原体。对于非沙眼衣原体性衣原体结膜炎患者，短期口服抗生素治疗似乎并不充分。肺炎衣原体和鹦鹉热衣原体引起的眼部感染可能比之前认为的更为常见，并且可以通过DFA和PCR进行鉴定。
