Kawakami M, Terai C, Okada Y
Department of Traumatology and Critical Care Medicine, National Defense Medical College, Tokorozawa, Saitama, Japan.
J Trauma. 1998 Jun;44(6):1056-63. doi: 10.1097/00005373-199806000-00019.
To delineate the regulation of IL-6 production in unburned skin adjacent to a burn in an animal model.
In C57BL/6 mice, at 15, 30, and 60 minutes after a 20% full-thickness burn, skin was removed from various sites. Control samples were obtained from unburned mice. Normal skins were incubated with tumor necrosis factor-alpha (TNF-alpha), interleukin-1 alpha (IL-1alpha), IL-1 beta, and IL-6. Unburned skin specimens were incubated with anti-TNF-alpha and IL-1alpha antibodies. Cytokine levels were measured by enzyme-linked immunosorbent assay.
The burn increased the IL-6 levels at 30 minutes (p < 0.05) and the IL-1alpha levels at 15 and 60 minutes in the unburned skin. TNF-alpha, IL-1alpha, and IL-1beta increased IL-6 production in normal skin (p < 0.05). Anti-IL-1alpha antibody decreased IL-6 production in the unburned skin (p < 0.05).
IL-1alpha modulates IL-6 production in unburned skin after injury. IL-6 and IL-1alpha might contribute to the alterations after a burn.
在动物模型中描绘烧伤附近未烧伤皮肤中白细胞介素-6（IL-6）产生的调节机制。
在C57BL/6小鼠中，20%全层烧伤后15、30和60分钟，从不同部位取下皮肤。对照样本取自未烧伤小鼠。将正常皮肤与肿瘤坏死因子-α（TNF-α）、白细胞介素-1α（IL-1α）、白细胞介素-1β（IL-1β）和白细胞介素-6一起孵育。将未烧伤的皮肤标本与抗TNF-α和IL-1α抗体一起孵育。通过酶联免疫吸附测定法测量细胞因子水平。
烧伤使未烧伤皮肤中30分钟时的IL-6水平升高（p<0.05），以及15和60分钟时的IL-1α水平升高。TNF-α、IL-1α和IL-1β增加正常皮肤中的IL-6产生（p<0.05）。抗IL-1α抗体降低未烧伤皮肤中的IL-6产生（p<0.05）。
IL-1α调节损伤后未烧伤皮肤中IL-6的产生。IL-6和IL-1α可能促成烧伤后的改变。
