热损伤后不同部位皮肤中白细胞介素-6水平的变化。

Changes of the interleukin-6 levels in skin at different sites after thermal injury.

作者信息

Kawakami M, Terai C, Okada Y

机构信息

Department of Traumatology and Critical Care Medicine, National Defense Medical College, Tokorozawa, Saitama, Japan.

出版信息

J Trauma. 1998 Jun;44(6):1056-63. doi: 10.1097/00005373-199806000-00019.

DOI:10.1097/00005373-199806000-00019

PMID:9637162

Abstract

OBJECTIVE

To delineate the regulation of IL-6 production in unburned skin adjacent to a burn in an animal model.

METHODS

In C57BL/6 mice, at 15, 30, and 60 minutes after a 20% full-thickness burn, skin was removed from various sites. Control samples were obtained from unburned mice. Normal skins were incubated with tumor necrosis factor-alpha (TNF-alpha), interleukin-1 alpha (IL-1alpha), IL-1 beta, and IL-6. Unburned skin specimens were incubated with anti-TNF-alpha and IL-1alpha antibodies. Cytokine levels were measured by enzyme-linked immunosorbent assay.

RESULTS

The burn increased the IL-6 levels at 30 minutes (p < 0.05) and the IL-1alpha levels at 15 and 60 minutes in the unburned skin. TNF-alpha, IL-1alpha, and IL-1beta increased IL-6 production in normal skin (p < 0.05). Anti-IL-1alpha antibody decreased IL-6 production in the unburned skin (p < 0.05).

CONCLUSIONS

IL-1alpha modulates IL-6 production in unburned skin after injury. IL-6 and IL-1alpha might contribute to the alterations after a burn.

摘要

目的

在动物模型中描绘烧伤附近未烧伤皮肤中白细胞介素-6（IL-6）产生的调节机制。

方法

在C57BL/6小鼠中，20%全层烧伤后15、30和60分钟，从不同部位取下皮肤。对照样本取自未烧伤小鼠。将正常皮肤与肿瘤坏死因子-α（TNF-α）、白细胞介素-1α（IL-1α）、白细胞介素-1β（IL-1β）和白细胞介素-6一起孵育。将未烧伤的皮肤标本与抗TNF-α和IL-1α抗体一起孵育。通过酶联免疫吸附测定法测量细胞因子水平。