Steinstraesser Lars, Burkhard Olaf, Fan Ming H, Jacobsen Frank, Lehnhardt Marcus, Su Grace, Daigeler Adrien, Steinau Hans U, Remick Daniel, Wang Stewart C
Dept. of Surgery, University of Michigan, Ann Arbor, MI 48109-0666, USA.
BMC Surg. 2005 Sep 17;5:19. doi: 10.1186/1471-2482-5-19.
Despite dramatic improvements in the management of burns, infection still remains a serious risk for the burn patient. The aim of this study was to shed light on the impact of acute burn injury with or without infection on cytokine profiles.
Sprague-Dawley rats (n = 21) were randomized into three groups: 1) burn only 2) burn and infection or 3) sham burn. Weight was monitored and blood was collected for cytokine ELISA, LPS quantification, and peripheral blood analysis. Animals were sacrificed either after 6 or 12 days.
Infected animals showed substantial weight loss until day 6 post-burn as compared to burn alone. Endotoxin and TNF-alpha levels were elevated early in the infected burn group within 48 hours post-burn. In contrast, significant up-regulation of the anti-inflammatory cytokine IL-10 occurred later in the clinical course and was associated with the recovery from weight loss.
Our results suggest that in the presence of infection, you get a SIRS response possibly due to transient endotoxemia that is only seen in the infection group. In contrast, both burn and infection get a late IL-10 (CARS) response, which is then associated with a return to normal weight in the infection group.
尽管烧伤治疗有了显著改善，但感染仍是烧伤患者面临的严重风险。本研究旨在阐明有无感染的急性烧伤对细胞因子谱的影响。
将21只Sprague-Dawley大鼠随机分为三组：1）仅烧伤组；2）烧伤合并感染组；3）假烧伤组。监测体重并采集血液进行细胞因子酶联免疫吸附测定（ELISA）、脂多糖（LPS）定量及外周血分析。在6天或12天后处死动物。
与仅烧伤组相比，感染组动物在烧伤后第6天前体重显著减轻。感染烧伤组在烧伤后48小时内，内毒素和肿瘤坏死因子-α（TNF-α）水平早期升高。相比之下，抗炎细胞因子白细胞介素-10（IL-10）在临床病程后期显著上调，且与体重减轻的恢复相关。
我们的结果表明，在存在感染的情况下，可能由于仅在感染组中出现的短暂内毒素血症而引发全身炎症反应综合征（SIRS）。相比之下，烧伤和感染均会引发后期的IL-10（代偿性抗炎反应综合征，CARS）反应，这与感染组体重恢复正常相关。
