Hilali M, Romand S, Thulliez P, Kwok O C, Dubey J P
Department of Parasitology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Vet Parasitol. 1998 Feb 28;75(2-3):269-71. doi: 10.1016/s0304-4017(97)00181-7.
Sera from camels from Egypt were examined by the direct agglutination tests incorporating mercaptoethanol for antibodies to Neospora caninum and Toxoplasma gondii. Antibodies to N. caninum were found in 6 of 161 camels in titers of 1:40 (2 camels) and 1:80, 1:160, 1:640, and 1:1280 in 1 camel each, using N. caninum formalin preserved whole tachyzoites as antigen. Antibodies to T. gondii were found in 17.4% of 166 camels in titers of 1:25 (3 camels), 1:50 (18 camels). and > 1:500 (8 camels) using T. gondii tachyzoites. All 6 camels with N. caninum antibodies had no T. gondii antibodies in 1:4 dilution of serum, indicating specificity of the reaction. This is the first report of N. caninum prevalence in Egypt.
利用含巯基乙醇的直接凝集试验，检测了来自埃及骆驼的血清中抗犬新孢子虫和抗刚地弓形虫的抗体。以犬新孢子虫福尔马林固定的全速殖子作为抗原，在161头骆驼中有6头检测到抗犬新孢子虫抗体，滴度分别为1:40（2头骆驼）、1:80、1:160、1:640和1:1280（各1头骆驼）。以刚地弓形虫速殖子作为抗原，在166头骆驼中有17.4%检测到抗刚地弓形虫抗体，滴度分别为1:25（3头骆驼）、1:50（18头骆驼）和>1:500（8头骆驼）。所有6头有抗犬新孢子虫抗体的骆驼在血清1:4稀释时均无抗刚地弓形虫抗体，表明反应具有特异性。这是埃及犬新孢子虫流行情况的首次报告。
