Shim Y S, Kim C W, Lee W K
Department of Biological Science, Myong Ji University, Yongin, Korea.
Mol Cells. 1998 Apr 30;8(2):226-32.
To reveal sequence variations in the Epstein-Barr virus nuclear antigen 2 (EBNA2) genes of Epstein-Barr virus (EBV) strains circulating in the Korean population, the EBNA2 divergent region was amplified and sequenced from 13 EBV-1 isolates, 2 EBNA2 type 1 intertypic EBV isolates, and two EBV-2 isolates, all derived from Korean cancer patients. Comparative sequence analysis revealed that type 1 and type 2 EBNA2 divergent regions of Korean EBV isolates were almost identical to the respective regions of the B95-8 EBV-1 and AG876 EBV-2 strains. Two of the nucleotide changes found in the type 1 divergent regions of all Korean isolates, G-to-T and C-to-A at B95-8 EBV positions 48,991 and 48,998, respectively, are also present in all EBV-1 strains of non-Asian origins, indicating that these two sites might be mutational hot sites. Besides these two mutations, EBV-1 strains from Papua New Guinea or European Caucasians and Africa show unique patterns of identical sequence variations from B95-8, which were not found in type 1 isolates from Korea, suggesting that Korean type 1 are evolving as a lineage distinct from isolates from Papua New Guinea or European Caucasian and Africa.
为了揭示在韩国人群中传播的爱泼斯坦-巴尔病毒（EBV）株的爱泼斯坦-巴尔病毒核抗原2（EBNA2）基因中的序列变异，对来自13株EBV-1分离株、2株EBNA2 1型种间EBV分离株和2株EBV-2分离株的EBNA2差异区域进行了扩增和测序，所有这些分离株均来自韩国癌症患者。比较序列分析显示，韩国EBV分离株的1型和2型EBNA2差异区域与B95-8 EBV-1和AG876 EBV-2株的相应区域几乎相同。在所有韩国分离株的1型差异区域中发现的两个核苷酸变化，即在B95-8 EBV位置48,991处的G到T和在位置48,998处的C到A，在所有非亚洲来源的EBV-1株中也存在，这表明这两个位点可能是突变热点。除了这两个突变外，来自巴布亚新几内亚或欧洲白种人和非洲的EBV-1株显示出与B95-8相同的独特序列变异模式，而在韩国的1型分离株中未发现，这表明韩国的1型正在作为一个与来自巴布亚新几内亚或欧洲白种人和非洲的分离株不同的谱系进化。
