Chen L, Foged N T
Department of Endocrinology, Xiehe Hospital, Tongji Medical University, Wuhan.
J Tongji Med Univ. 1997;17(2):72-4. doi: 10.1007/BF02888237.
The effect of progesterone on osteocalcin gene expression was investigated in order to understand how progesterone acts on osteoblast in vitro. For this purpose, fetal rat calvarial osteoblasts were cultured in the medium containing 10(-9) M-10(-6) M progesterone respectively for two weeks while the cells progressing differentiation. Northern blot analysis showed that progesterone increased the level of osteocalcin mRNA in a dose-dependent manner. Among those doses of progesterone employed in this study, only 10(-6) M hormone caused a significant induction of osteocalcin gene expression (2.3 fold, P < 0.01). Our results demonstrated for the first time that the osteocalcin gene expression is regulated by progesterone. In addition, the results obtained from our study provides a good evidence for the direct effect of progesterone on osteoblast in vitro.
为了解孕酮在体外如何作用于成骨细胞，研究了孕酮对骨钙素基因表达的影响。为此，将胎鼠颅骨成骨细胞分别在含有10⁻⁹M - 10⁻⁶M孕酮的培养基中培养两周，同时细胞进行分化。Northern印迹分析表明，孕酮以剂量依赖的方式增加骨钙素mRNA水平。在本研究使用的那些孕酮剂量中，只有10⁻⁶M激素引起骨钙素基因表达的显著诱导（2.3倍，P < 0.01）。我们的结果首次证明骨钙素基因表达受孕酮调节。此外，我们研究获得的结果为孕酮在体外对成骨细胞的直接作用提供了有力证据。
