Abe T, Nawa Y
Department of Parasitology, Miyazaki Medical College, Japan.
Parasitol Res. 1988;74(5):484-8. doi: 10.1007/BF00535150.
Mast-cell growth factor (MCGF) activity in the media conditioned by mesenteric lymph node or spleen cells from Strongyloides ratti-infected C57BL/6 mice was examined by using factor-dependent cell line FDC-P2 or bone marrow-derived, cultured mast cells (BMMC) as indicators. Mesenteric lymph node cells from infected mice spontaneously released MCGF activity by culturing for 24 h, showing peak production on days 5-7. MCGF production by mesenteric lymph node cells was augmented after stimulation with adult worm antigen or with concanavalin A (con A). The peak of MCGF production by antigen-stimulated lymph node cells was observed on days 5-7 and declined thereafter. MCGF production by antigen-stimulated spleen cells was lower than that by lymph node cells and reached a peak on day 7 or later. Normal lymph node or spleen cells did not produce MCGF activity even after stimulation with adult worm antigen. The peak of MCGF production by mesenteric lymph node cells preceded the peak of intestinal mastocytosis at the infected site by 4-6 days. The cells producing MCGF had a phenotype of Thy-1+, L3T4+, and Lyt-2-. The possible importance of mucosal mast cells in worm expulsion is discussed.
利用依赖因子的细胞系FDC - P2或骨髓来源的培养肥大细胞（BMMC）作为指标，检测了来自感染大鼠类圆线虫的C57BL/6小鼠肠系膜淋巴结或脾细胞所产生的培养基中的肥大细胞生长因子（MCGF）活性。感染小鼠的肠系膜淋巴结细胞通过培养24小时可自发释放MCGF活性，在第5 - 7天产量达到峰值。用成虫抗原或伴刀豆球蛋白A（cona）刺激后，肠系膜淋巴结细胞的MCGF产量增加。抗原刺激的淋巴结细胞产生MCGF的峰值在第5 - 7天出现，随后下降。抗原刺激的脾细胞产生的MCGF低于淋巴结细胞，且在第7天或更晚达到峰值。正常淋巴结或脾细胞即使在用成虫抗原刺激后也不产生MCGF活性。肠系膜淋巴结细胞产生MCGF的峰值比感染部位肠道肥大细胞增多的峰值提前4 - 6天。产生MCGF的细胞具有Thy - 1 +、L3T4 +和Lyt - 2 -的表型。本文讨论了黏膜肥大细胞在驱蠕虫过程中可能的重要性。
