类风湿关节炎患者滑液中抗凝血酶III的失活
Inactivation of antithrombin III in synovial fluid from patients with rheumatoid arthritis.
作者信息
Jones H W, Bailey R, Zhang Z, Dunne K A, Blake D R, Cox N L, Morris C J, Winyard P G
机构信息
Department of Rheumatology, Royal Hampshire County Hospital, Winchester.
出版信息
Ann Rheum Dis. 1998 Mar;57(3):162-5. doi: 10.1136/ard.57.3.162.
OBJECTIVE
To investigate the thrombin inhibitory capacity of antithrombin III in the inflamed human joint.
METHODS
Thrombin inhibitory capacity was measured, using a kinetic spectophotometric method, in matched plasma and synovial fluid samples of patients with rheumatoid arthritis (n = 22) and osteoarthritis (n = 16), together with normal control plasma samples (n = 13). In the same samples, the concentration of antithrombin III was also determined by the method of radial immunodiffusion. The combination of these measurements allowed the calculation of the specific thrombin inhibitory capacity of these samples.
RESULTS
An increased concentration of antithrombin III in rheumatoid compared with osteoarthritic synovial fluid was noted (p < 0.05). However, there was a significant depression in the specific activity of antithrombin III in rheumatoid synovial fluid when compared with matched plasma samples (p < 0.001) or with osteoarthritic synovial fluid (p < 0.05).
CONCLUSION
In rheumatoid synovial fluid the thrombin inhibitory capacity of antithrombin III is disproportionately depressed relative to the concentration of antithrombin III, indicating the inactivation of antithrombin III in the rheumatoid joint.
目的
研究抗凝血酶III在人类炎症关节中的凝血酶抑制能力。
方法
采用动力学分光光度法，对类风湿关节炎患者（n = 22）、骨关节炎患者（n = 16）的配对血浆和滑液样本以及正常对照血浆样本（n = 13）进行凝血酶抑制能力测定。同时，采用放射免疫扩散法测定相同样本中抗凝血酶III的浓度。通过这些测量结果可计算出这些样本的特异性凝血酶抑制能力。
结果
与骨关节炎滑液相比，类风湿关节炎滑液中抗凝血酶III的浓度升高（p < 0.05）。然而，与配对血浆样本相比（p < 0.001）或与骨关节炎滑液相比（p < 0.05），类风湿关节炎滑液中抗凝血酶III的比活性显著降低。
结论
在类风湿关节炎滑液中，抗凝血酶III的凝血酶抑制能力相对于抗凝血酶III的浓度不成比例地降低，表明类风湿关节中抗凝血酶III失活。
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