Ninomiya Y, Imoto T, Yatabe A, Kawamura S, Nakashima K, Katsukawa H
Department of Oral Physiology, Chemistry and Pediatric Dentistry, Asahi University School of Dentistry, Gifu, Japan.
Am J Physiol. 1998 May;274(5):R1324-30. doi: 10.1152/ajpregu.1998.274.5.R1324.
Genetically diabetic db/db mice show greater neural and behavioral responses to sugars than lean control mice. The present study examined chorda tympani responses of db/db mice to nonsugar sweeteners and their inhibition by a sweet response inhibitor, gurmarin. The results showed that responses to sucrose, saccharin, glycine, L-alanine, and D-tryptophan, but not to D-phenylalanine, were approximately 1.5 times greater in db/db mice than in control mice. Treatment of the tongue with gurmarin suppressed responses to these sweeteners in db/db and control mice, but the extent of suppression was considerably smaller in db/db mice. The magnitudes of gurmarin-sensitive components of the response to sweeteners in db/db mice were not significantly different from those in control mice, whereas the magnitudes of gurmarin-insensitive components in db/db mice were about twice as large as those in control mice. These results suggest that the enhancement of chorda tympani responses in db/db mice to sucrose and other nonsugar sweeteners may occur through gurmarin-insensitive membrane components.
遗传性糖尿病db/db小鼠对糖类的神经和行为反应比瘦型对照小鼠更强烈。本研究检测了db/db小鼠的鼓索神经对非糖类甜味剂的反应以及甜味反应抑制剂古马隆对其的抑制作用。结果显示，db/db小鼠对蔗糖、糖精、甘氨酸、L-丙氨酸和D-色氨酸（而非D-苯丙氨酸）的反应比对照小鼠大约高1.5倍。用古马隆处理舌头可抑制db/db小鼠和对照小鼠对这些甜味剂的反应，但db/db小鼠的抑制程度要小得多。db/db小鼠对甜味剂反应中对古马隆敏感成分的大小与对照小鼠无显著差异，而db/db小鼠中对古马隆不敏感成分的大小约为对照小鼠的两倍。这些结果表明，db/db小鼠鼓索神经对蔗糖和其他非糖类甜味剂反应的增强可能是通过对古马隆不敏感的膜成分实现的。
