Wang M B, Cheng Z, Keese P, Graham M W, Larkin P J, Waterhouse P M
CRC for Plant Science, Canberra, Australia.
Arch Virol. 1998;143(5):1005-13. doi: 10.1007/s007050050349.
Barely yellow dwarf luteovirus-GPV (BYDV-GPV) is a common problem in Chinese wheat crops but is unrecorded elsewhere. A defining characteristic of GPV is its capacity to be transmitted efficiently by both Schizaphis graminum and Rhopaloshiphum padi. This dual aphid species transmission contrasts with those of BYDV-RPV and BYDV-SGV, globally distributed viruses, which are efficiently transmitted only by Rhopaloshiphum padi and Schizaphis graminum respectively. The viral RNA sequences encoding the coat protein (22K) gene, the movement protein (17K) gene, the region surrounding the conserved GDD motif of the polymerase gene and the intergenic sequences between these genes were determined for GPV and an Australian isolate of BYDV-RPV (RPVa). In all three genes, the sequences of GPV and RPVa were more similar to those of an American isolate of BYDV-RPV (RPVu) than to any other luteovirus for which there is data available. RPVa and RPVu were very similar, especially their coat proteins which had 97% identity at the amino acid level. The coat protein of GPV had 76% and 78% amino acid identity with RPVa and RPVu respectively. The data suggest that RPVu and RPVa are correctly named as strains of the same serotype and that GPV is sufficiently different from either RPV strain to be considered a distinct BYDV type. The coat protein and movement protein genes of GPV are very dissimilar to SGV. The polymerase sequences of RPVu, RPVa and GPV show close affinities with those of the sobemo-like luteoviruses and little similarity with those of the carmo-like luteoviruses. The sequences of the coat proteins, movement proteins and the polymerase segments of BYDV serotypes, other than RPV and GPV, form a cluster that is separate from their counterpart sequences from dicot-infecting luteoviruses. The RPV and GPV isolates consistently fall within a dicot-infecting cluster. This suggests that RPV and GPV evolved from within this group of viruses. Since these other viruses all infect dicots it seems likely that their common ancestor infected a dicot and that RPV and GPV evolved from a virus that switched hosts from a dicot to a monocot.
大麦黄矮病毒 - GPV（BYDV - GPV）是中国小麦作物中的常见问题，但在其他地方尚无记录。GPV的一个显著特征是它能够被麦二叉蚜和禾谷缢管蚜高效传播。这种由两种蚜虫传播的特性与全球分布的BYDV - RPV和BYDV - SGV不同，后两种病毒分别仅由禾谷缢管蚜和麦二叉蚜高效传播。测定了编码GPV外壳蛋白（22K）基因、运动蛋白（17K）基因、聚合酶基因保守GDD基序周围区域以及这些基因之间基因间隔序列的病毒RNA序列，以及澳大利亚分离的BYDV - RPV（RPVa）的相应序列。在所有三个基因中，GPV和RPVa的序列与美国分离的BYDV - RPV（RPVu）的序列比与其他任何有数据的黄矮病毒的序列更相似。RPVa和RPVu非常相似，尤其是它们的外壳蛋白在氨基酸水平上有97%的同一性。GPV的外壳蛋白与RPVa和RPVu的氨基酸同一性分别为76%和78%。数据表明，RPVu和RPVa被正确地命名为同一血清型的毒株，并且GPV与任何一种RPV毒株都有足够的差异，应被视为一种不同的BYDV类型。GPV的外壳蛋白和运动蛋白基因与SGV非常不同。RPVu、RPVa和GPV的聚合酶序列与类 Sobemo 黄矮病毒的聚合酶序列有密切的亲缘关系，与类 Carmo 黄矮病毒的聚合酶序列几乎没有相似性。除RPV和GPV外，BYDV血清型的外壳蛋白、运动蛋白和聚合酶片段的序列形成一个与感染双子叶植物的黄矮病毒对应序列分开的簇。RPV和GPV分离株始终落在感染双子叶植物的簇内。这表明RPV和GPV是从这组病毒中进化而来的。由于这些其他病毒都感染双子叶植物，它们的共同祖先似乎感染了双子叶植物，并且RPV和GPV是从一种将宿主从双子叶植物转换为单子叶植物的病毒进化而来的。
