大麦黄矮病毒类RMV株系的外壳蛋白序列使其与其他黄症病毒区分开来。

Coat protein sequences of RMV-like strains of barley yellow dwarf virus separate them from other luteoviruses.

作者信息

Domier L L, Lukasheva L I, D'Arcy C J

机构信息

Department of Plant Pathology, University of Illinois, Urbana 61801.

出版信息

Intervirology. 1994;37(1):2-5. doi: 10.1159/000150347.

DOI:10.1159/000150347

PMID:7928284

Abstract

Illinois (IL) and Minnesota (MN) RMV-like strains of barley yellow dwarf virus (BYDV) were identified from maize displaying red leaf symptoms by enzyme-linked immunosorbent assay (ELISA) using antiserum against a New York strain (BYDV-RMV-NY). Some IL and MN strains, but not the NY strain, could be detected by ELISA with a monoclonal antibody raised against BYDV-RPV-NY. The region of the viral genome representing the coat protein gene was amplified by polymerase chain reaction, cloned and sequenced. The nucleotide sequences of the BYDV-RMV-IL and BYDV-RMV-MN coat protein genes differed at just five nucleotide positions while the BYDV-RMV-IL and BYDV-RMV-NY differed at 101 of the 591 positions. The predicted amino acid sequences of the coat proteins of RMV-like strains from IL, MN, and NY shared approximately 60% identify with those of the coat proteins of beet western yellows virus, BYDV-RPV-NY, and potato leafroll virus.

摘要

通过使用针对纽约株系（大麦黄矮病毒-RMV-NY）的抗血清进行酶联免疫吸附测定（ELISA），从表现出红叶症状的玉米中鉴定出伊利诺伊州（IL）和明尼苏达州（MN）的类大麦黄矮病毒-RMV株系。一些伊利诺伊州和明尼苏达州的株系，但不是纽约株系，可以通过使用针对大麦黄矮病毒-RPV-NY产生的单克隆抗体进行ELISA检测到。通过聚合酶链反应扩增、克隆并测序代表病毒基因组外壳蛋白基因的区域。大麦黄矮病毒-RMV-IL和大麦黄矮病毒-RMV-MN外壳蛋白基因的核苷酸序列仅在五个核苷酸位置不同，而大麦黄矮病毒-RMV-IL和大麦黄矮病毒-RMV-NY在591个位置中的101个位置不同。来自伊利诺伊州、明尼苏达州和纽约州的类RMV株系外壳蛋白的预测氨基酸序列与甜菜西方黄化病毒、大麦黄矮病毒-RPV-NY和马铃薯卷叶病毒外壳蛋白的氨基酸序列具有约60%的同一性。