斑点叉尾鮰皮肤中的抗菌活性：广谱组蛋白样抗菌蛋白的特性
Antimicrobial activity in the skin of the channel catfish Ictalurus punctatus: characterization of broad-spectrum histone-like antimicrobial proteins.
作者信息
Robinette D, Wada S, Arroll T, Levy M G, Miller W L, Noga E J
机构信息
Department of Companion Animal and Special Species Medicine, College of Veterinary Medicine, North Carolina State University, Raleigh 27606, USA.
出版信息
Cell Mol Life Sci. 1998 May;54(5):467-75. doi: 10.1007/s000180050175.
Abstract
Three antibacterial proteins were isolated from acid extracts of channel catfish (Ictalurus punctatus) skin by cation exchange chromatography and reverse-phase high-pressure liquid chromatography. The molecular masses of the proteins were 15.5, 15.5 and 30 kD as determined by SDS-polyacrylamide gel electrophoresis. Mass spectrometry, amino acid composition and amino acid sequence data suggest that the most abundant protein is closely related to histone H2B. The H2B-like protein was inhibitory to Aeromonas hydrophila and Saprolegnia spp., which are important bacterial and fungal pathogens of fish. These findings suggest that histones may be important defensive molecules in fish.
摘要
通过阳离子交换色谱法和反相高压液相色谱法，从斑点叉尾鮰（Ictalurus punctatus）皮肤的酸性提取物中分离出三种抗菌蛋白。经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定，这些蛋白质的分子量分别为15.5、15.5和30千道尔顿。质谱分析、氨基酸组成和氨基酸序列数据表明，含量最丰富的蛋白质与组蛋白H2B密切相关。这种类H2B蛋白对嗜水气单胞菌和水霉属具有抑制作用，嗜水气单胞菌和水霉属分别是鱼类重要的细菌和真菌病原体。这些发现表明，组蛋白可能是鱼类重要的防御分子。
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