Nemcová R, Bomba A, Herich R, Gancarcíková S
Research Institute of Experimental Veterinary Medicine, Kosice, Slovak Republic.
Dtsch Tierarztl Wochenschr. 1998 May;105(5):199-200.
In the present study, the effect of Lactobacillus casei subsp. casei and Lactobacillus fermentum inoculation on jejunum and ileum colonization in gnotobiotic piglets has been observed. The characteristic features of the strains used were strong adherence to pig epithelial gut cells as well as inhibitory activity against enteropathogenic E. coli under in vitro conditions. Strains were inoculated to 2, 3, and 4 day old gnotobiotic piglets at a dose of 2 ml (1 x 10(8) germs/ml). On the second day after the last inoculation, Lactobacillus casei subsp. casei strain counts adhered to the jejunum mucosa and those adhered to the ileum mucosa were 4.54 log 10.cm-2 and 5.40 log 10.cm-2, respectively. Lactobacillus fermentum counts adhered to the jejunum mucosa and those adhered to the ileum mucosa were 5.73 log 10.cm-2 and 4.01 log 10.cm-2, respectively. On day 5 after the last inoculation, the counts in both strains were by one log higher. The results obtained point out to the fact that Lactobacillus casei subsp. casei and Lactobacillus fermentum colonized the mucosa of both jejunum and ileum and survived in the intestinal tract. The adherence of lactobacilli to gut cells in vitro correlated with their capability to adhere to the mucosa of both jejunum and ileum in vivo.
在本研究中，观察了干酪乳杆菌干酪亚种和发酵乳杆菌接种对无菌仔猪空肠和回肠定植的影响。所用菌株的特征是在体外条件下对猪肠道上皮细胞有很强的黏附性以及对肠道致病性大肠杆菌有抑制活性。将菌株以2 ml（1×10⁸ 个菌/ml）的剂量接种到2、3和4日龄的无菌仔猪。在最后一次接种后的第二天，干酪乳杆菌干酪亚种黏附于空肠黏膜的菌株计数和黏附于回肠黏膜的菌株计数分别为4.54 log₁₀.cm⁻² 和5.40 log₁₀.cm⁻²。发酵乳杆菌黏附于空肠黏膜的计数和黏附于回肠黏膜的计数分别为5.73 log₁₀.cm⁻² 和4.01 log₁₀.cm⁻²。在最后一次接种后的第5天，两种菌株的计数均高出一个对数级。所得结果表明，干酪乳杆菌干酪亚种和发酵乳杆菌在空肠和回肠黏膜定植并在肠道内存活。乳酸菌在体外对肠道细胞的黏附与其在体内对空肠和回肠黏膜的黏附能力相关。
