Bomba A, Gancarcíková S, Nemcová R, Herich R, Kastel R, Depta A, Demeterová M, Ledeck V, Zitnan R
Research Institute of Experimental Veterinary Medicine, Kosice, Slovak Republic.
Dtsch Tierarztl Wochenschr. 1998 Oct;105(10):384-9.
The effect of inoculation of Lactobacillus casei on selected parameters of metabolic profile and intestinal metabolism of gnotobiotic piglets was investigated during the first three weeks of their life. The experiment was carried out on 8 germ-free piglets. The experimental group was inoculated once a day with the Lactobacillus casei subsp. casei strain. The inoculum contained 1 x 10(8) microorganisms in 1 ml. The control group of piglets received no inoculum. Lactobacillus casei colonized jejunum and ileum in the numbers from 5.63 to 6.06 log 10 cm-2 and their numbers in the jejunal and ileal contents were in the range 8.38-9.87 log 10.ml-1. The daily consumption of milk by the inoculated animals was significantly higher (p < 0.001). The average weight of inoculated piglets at the end of the period investigated was higher by 29.7%. Lactobacillus casei affected several parameters investigated. Piglets inoculated with lactobacilli showed significantly lower (p < 0.05-0.01) values of pH of the jejunal content, numbers of erythrocytes, values of haematocrit, urea, glucose, total lipids, cholesterol and calcium in the serum and significantly higher values (p < 0.05-0.01) of lactic acid in the jejunal content. The values of phagocytic activity and the index of phagocytic activity in the piglets of the experimental group were two to three-fold higher in comparison with those detected in the control group. The application of Lactobacillus casei affected positively the growth of gnotobiotic piglets, their intestinal metabolism, the level of cholesterol in the serum and phagocytic activity.
在无菌仔猪出生后的前三周，研究了接种干酪乳杆菌对其代谢谱和肠道代谢选定参数的影响。实验选用了8只无菌仔猪。实验组每天接种一次干酪乳杆菌干酪亚种菌株。接种物每1毫升含1×10⁸个微生物。对照组仔猪不接种。干酪乳杆菌在空肠和回肠定殖，数量为5.63至6.06 log₁₀ cm⁻²，其在空肠和回肠内容物中的数量范围为8.38 - 9.87 log₁₀.ml⁻¹。接种动物的每日牛奶摄入量显著更高（p < 0.001）。在研究期末，接种仔猪的平均体重高出29.7%。干酪乳杆菌影响了所研究的几个参数。接种乳杆菌的仔猪空肠内容物pH值、红细胞数量、血清中血细胞比容、尿素、葡萄糖、总脂质、胆固醇和钙的值显著更低（p < 0.05 - 0.01），而空肠内容物中乳酸的值显著更高（p < 0.05 - 0.01）。实验组仔猪的吞噬活性值和吞噬活性指数比对照组检测到的值高出两到三倍。应用干酪乳杆菌对无菌仔猪的生长、肠道代谢、血清胆固醇水平和吞噬活性有积极影响。
