Bomba A, Nemcová R, Kastel R, Herich R, Pataky J, Cízek M
Research Institute of Experimental Veterinary Medicine, Kosice, Slovak Republic.
Vet Med (Praha). 1996 May;41(5):155-8.
In the present study, the effect of Lactobacillus spp. against enteropathogenic Escherichia coli 08:K88+Ent+ under in vitro and in vivo conditions has been compared and the effect of inoculation of Lactobacillus spp. upon the colonization of both the jejunum and ileum by enteropathogenic E. coli 08:K88+Ent+ in 9 gnotobiotic pigs has been observed. Under in vitro conditions, the strain Lactobacillus spp. showed the inhibition of 2.1 +/- 0.1 mm against enteropathogenic E. coli 08:K88+Ent+. Two days after the inoculation, the enteropathogenic E. coli 08:K88+Ent+, inoculated to the control group of gnotobiotic pigs (E), colonized the mucosa of both jejunum and ileum of gnotobiotic pigs at counts of 6.41 and 6.08 log 10/cm2, respectively. In experimental group (L-E), the counts of adhered enteropathogenic E. coli in the identical sections of the small intestine, following the inoculation by Lactobacillus spp., amounted to 6.35 and 6.43 log/cm2, respectively. In both groups, numbers of E. coli in the intestinal content of both jejunum and ileum were nearly the same (group E 9.03 and 9.31 log 10/ml; group L-E 8.97 and 9.11 log 10/ml). Two to five days after E. coli inoculation, Lactobacillus spp. counts adhered to the jejunal wall ranged from 5.4 to 6.49 log 10 cm2; in the ileum they ranged from 6.05 to 6.77 log 10 cm2. In the jejunal content, the lactobacilli counts ranged from 6.81 to 8.86 log 10/ml and in the ileum from 8.5 to 898 log 10/ml. Two days after the E. coli inoculation, the concentration of lactic acid in the content of jejunum in gnotobiotic pigs of the groups E and L-E was 16.3 mmol/l and 23.6 mmol/l, respectively. The concentration of acetic acid in the jejunum of the pigs of E and L-E groups was 15.9 mmol/l and 19.6 mmol/l, respectively. Similarly, the higher concentrations of both acids were found also in the ileum of the L-E pigs. The results obtained indicate that the used strain of Lactobacillus spp. which has been preventively inoculated to gnotobiotic pigs, did not prevent the adhesion of enteropathogenic E. coli 08:K88+Ent+ to the mucosa of both jejunum and ileum also despite of the demonstrated inhibitory effect against enteropathogenic E. coli under in vitro conditions and despite good adherent ability in vivo. In both groups of animals, the diseases with pronounced clinical signs as well as losses have occurred.
在本研究中，比较了乳酸杆菌属在体外和体内条件下对肠致病性大肠杆菌08:K88+Ent+的作用，并观察了在9头无菌猪中接种乳酸杆菌属对肠致病性大肠杆菌08:K88+Ent+在空肠和回肠定殖的影响。在体外条件下，乳酸杆菌属菌株对肠致病性大肠杆菌08:K88+Ent+显示出2.1±0.1毫米的抑制圈。接种两天后，接种到无菌猪对照组（E组）的肠致病性大肠杆菌08:K88+Ent+分别以6.41和6.08 log10/cm2的数量定殖于无菌猪空肠和回肠的黏膜。在实验组（L-E组）中，接种乳酸杆菌属后，小肠相同节段中黏附的肠致病性大肠杆菌数量分别为6.35和6.43 log/cm2。在两组中，空肠和回肠肠内容物中的大肠杆菌数量几乎相同（E组为9.03和9.31 log10/ml；L-E组为8.97和9.11 log10/ml）。大肠杆菌接种后2至5天，黏附于空肠壁的乳酸杆菌属数量在5.4至6.49 log10 cm2之间；在回肠中，数量在6.05至6.77 log10 cm2之间。在空肠内容物中，乳酸杆菌数量在6.81至8.86 log10/ml之间，在回肠中为8.5至898 log10/ml。大肠杆菌接种两天后，E组和L-E组无菌猪空肠内容物中乳酸的浓度分别为16.3 mmol/l和23.6 mmol/l。E组和L-E组猪空肠中乙酸的浓度分别为15.9 mmol/l和19.6 mmol/l。同样，在L-E组猪的回肠中也发现了两种酸的较高浓度。所得结果表明，预先接种到无菌猪的所用乳酸杆菌属菌株，尽管在体外条件下对肠致病性大肠杆菌有明显的抑制作用，且在体内有良好的黏附能力，但并未阻止肠致病性大肠杆菌08:K88+Ent+黏附于空肠和回肠的黏膜。在两组动物中，都出现了具有明显临床症状和损失的疾病。
