腐败希瓦氏菌锰（IV）还原缺陷突变体分离的两种快速筛选技术的设计与应用
Design and Application of Two Rapid Screening Techniques for Isolation of Mn(IV) Reduction-Deficient Mutants of Shewanella putrefaciens.
作者信息
Burnes BS, Mulberry MJ, DiChristina TJ
机构信息
School of Biology, Georgia Institute of Technology, Atlanta, Georgia 30332.
出版信息
Appl Environ Microbiol. 1998 Jul 1;64(7):2716-20. doi: 10.1128/AEM.64.7.2716-2720.1998.
Abstract
Chemical mutagenesis procedures and two newly developed rapid plate assays were used to identify two Mn(IV) reduction-deficient (Mnr) mutants of Shewanella putrefaciens. All eleven members of a set of previously isolated Fe(III) reduction-deficient (Fer) mutants displayed Mnr-positive phenotypes on the plate assays and were also capable of anaerobic growth on Mn(IV) as the sole terminal electron acceptor.
摘要
采用化学诱变程序和两种新开发的快速平板检测法，鉴定出腐败希瓦氏菌的两个锰（IV）还原缺陷（Mnr）突变体。一组先前分离出的铁（III）还原缺陷（Fer）突变体中的所有11个成员，在平板检测中均表现出Mnr阳性表型，并且也能够以锰（IV）作为唯一的末端电子受体进行厌氧生长。
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