Lincoln C K, Gabridge M G
Bionique Testing Laboratories, Inc., Saranac Lake, New York 12983, USA.
Methods Cell Biol. 1998;57:49-65. doi: 10.1016/s0091-679x(08)61571-x.
The subject of the chapter is cell culture contamination. Contamination may enter the cell culture system as a physical, chemical, and/or biological component of the environment. The potential sources and consequences of cell culture contamination are unique to the cell culture system and the contaminant. A basic understanding of cell culture contamination is necessary to appreciate the need to develop and practice standardized cell culture procedures. General sources, consequences, and preventative measures are discussed for physical and chemical contamination based on current technology. Mycoplasmal contamination is the focus of the discussion on biological contamination and its impact on cell cultures. The introduction of other biological contaminants should be controlled by the institution of cell culture management procedures needed to minimize the incidence of mycoplasmal contamination. The need to eliminate the routine use of antibiotics in cell culture systems and institute routine testing to detect contamination is emphasized. More rapid detection of contamination should reduce the incidence of cross-contamination and minimize the consequences of any contamination event.
本章的主题是细胞培养污染。污染可能作为环境的物理、化学和/或生物成分进入细胞培养系统。细胞培养污染的潜在来源和后果因细胞培养系统和污染物而异。对细胞培养污染有基本的了解，对于认识到制定和实施标准化细胞培养程序的必要性是很有必要的。基于当前技术，讨论了物理和化学污染的一般来源、后果及预防措施。支原体污染是生物污染及其对细胞培养影响讨论的重点。其他生物污染物的引入应通过建立细胞培养管理程序来控制，以尽量减少支原体污染的发生率。强调了在细胞培养系统中消除常规使用抗生素并进行常规检测以检测污染的必要性。更快地检测污染应能减少交叉污染的发生率，并将任何污染事件的后果降至最低。
