Monteseirín J, Llamas E, Muñoz F, Bandrés F, Bono M J, Conde J
Servico Regional de Inmunología y Alergia, Hospital Universitario Virgen Macarena, Sevilla, Spain.
Allergol Immunopathol (Madr). 1993 Mar-Apr;21(2):75-8.
The low-affinity receptor IgE (Fc epsilon RII) as shown to be identical to CD23 several years ago. The presence of the CD23 was demonstrated on a variety of human cells, such as T cells, monocytes, eosinophils, platelets, lymphocytes B, etc., and it can be cleaved in soluble fragments. We studied the in vitro production of soluble CD23 (sCD23) in PBMC of patients with bronchial asthma and in healthy donor cells. If we stimulate production with a polyclonal activator [Phytohemagglutinin (PHA) at a concentration of 10 micrograms/ml], it can be seen that the greatest amounts of sCD23 are produced on the fourth day and that production is greater in asthmatic patients than in control group (p < 0.01). When stimulated additionally by an antigen, the production of sCD23: Is greater when stimulated with PHA. Is released in quantities greater than in healthy group cells, when the PBMC are stimulated with the antigen to which the patients are sensitive (olive pollen). The quantity released depends on the concentration of the antigen added to the culture. Is not released in greater quantities by healthy groups cells in the presence of the antigen than in healthy group cells without the antigen. Is not released in greater quantities by cells of asthmatics in the presence of an antigen to which they are not sensitive (D. pteronyssinus). This leads us to conclude that the release of sCD23 in these patients could play a role in the physiopathology of extrinsic bronchial asthma.
低亲和力受体IgE（FcεRII）在数年前被证明与CD23相同。已证实在多种人类细胞上存在CD23，如T细胞、单核细胞、嗜酸性粒细胞、血小板、B淋巴细胞等，并且它可以被切割成可溶性片段。我们研究了支气管哮喘患者外周血单个核细胞（PBMC）和健康供体细胞中可溶性CD23（sCD23）的体外产生情况。如果用多克隆激活剂[浓度为10微克/毫升的植物血凝素（PHA）]刺激产生，可见在第四天产生的sCD23量最大，且哮喘患者的产生量大于对照组（p<0.01）。当用抗原额外刺激时，sCD23的产生情况如下：在用PHA刺激时产生量更大。当PBMC用患者敏感的抗原（橄榄花粉）刺激时，释放量大于健康组细胞。释放量取决于添加到培养物中的抗原浓度。在有抗原存在时，健康组细胞释放的量并不比没有抗原时的健康组细胞释放量更大。在有它们不敏感的抗原（屋尘螨）存在时，哮喘患者的细胞释放量不会更大。这使我们得出结论，这些患者中sCD23的释放可能在外源性支气管哮喘的病理生理过程中起作用。
