Daba M H, Abdel-Rahman M S
University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Pharmacology and Physiology Department, Newark 07103-2714, USA.
Toxicol Lett. 1998 Mar 16;95(1):23-9. doi: 10.1016/s0378-4274(98)00012-5.
Thymoquinone, the active constituent of Nigella sativa, was tested in isolated rat hepatocytes as a hepatoprotective agent against tert-butyl hydroperoxide (TBHP) toxicity. TBHP (2 mM) was used to produce oxidative injury in isolated rat hepatocytes and caused progressive depletion of intracellular glutathione (GSH), loss of cell viability as evidenced by trypan blue uptake and leakage of cytosolic enzymes, alanine transaminase (ALT) and aspartic transaminase (AST). Preincubation of hepatocytes with 1 mM of either thymoquinone or silybin, which is a known hepatoprotective agent, resulted in the protection of isolated hepatocytes against TBHP induced toxicity evidenced by decreased leakage of ALT and AST, and by decreased trypan blue uptake in comparison to TBHP treated hepatocytes. Both thymoquinone and silybin prevented TBHP induced depletion of GSH to the same extent. Although thymoquinone protected the liver enzymes leakage, the degree of protection was less than that caused by silybin.
黑种草籽的活性成分百里醌，作为一种抗叔丁基过氧化氢（TBHP）毒性的肝保护剂，在分离的大鼠肝细胞中进行了测试。TBHP（2 mM）用于在分离的大鼠肝细胞中产生氧化损伤，并导致细胞内谷胱甘肽（GSH）逐渐消耗，细胞活力丧失，这可通过台盼蓝摄取以及胞质酶丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）的泄漏来证明。用1 mM百里醌或水飞蓟宾（一种已知的肝保护剂）对肝细胞进行预孵育，结果显示分离的肝细胞对TBHP诱导的毒性具有保护作用，这可通过与TBHP处理的肝细胞相比，ALT和AST泄漏减少以及台盼蓝摄取减少来证明。百里醌和水飞蓟宾均在相同程度上防止了TBHP诱导的GSH消耗。尽管百里醌保护了肝酶泄漏，但其保护程度低于水飞蓟宾所引起的保护程度。
