Kowalski J
Department of Clinical Pharmacology, Silesian University School of Medicine, Katowice, Poland.
Eur J Pharmacol. 1998 Apr 17;347(1):95-9. doi: 10.1016/s0014-2999(98)00079-x.
The aim of this study was to evaluate the effects of [Met5]enkephalin, des-Met-[Met5]enkephalin, des-Tyr-[Met5]enkephalin, and Tyr-Gly-Gly on natural killer cytotoxic activity and on concanavalin A- and lipopolysaccharide-stimulated proliferation of B and T cells in mice. Single i.p. injections of [Met5]enkephalin, des-Met-[Met5]enkephalin, and Tyr-Gly-Gly increased both natural killer cytotoxicity and proliferation of mitogen-stimulated B and T cells. These effects were inhibited by naloxone pretreatment, which suggests the opioid mechanism of the peptides studied. The rate of lymphocytic proliferation increases not only after single injection of [Met5]enkephalin or its metabolites, but also after 3 or 7 days of treatment. Apart from the functional effects, [Met5]enkephalin, des-Met-[Met5]enkephalin, and Tyr-Gly-Gly increased the percentages of natural killer cells and T cells. The results of this study suggest that the immunomodulatory action of [Met5]enkephalin may be mediated or enhanced by its N-terminal metabolites des-Met-[Met5]enkephalin and Tyr-Gly-Gly.
本研究的目的是评估[Met5]脑啡肽、去甲硫氨酸-[Met5]脑啡肽、去酪氨酸-[Met5]脑啡肽和酪氨酰-甘氨酰-甘氨酸对小鼠自然杀伤细胞细胞毒性活性以及对刀豆蛋白A和脂多糖刺激的B细胞和T细胞增殖的影响。腹腔注射一次[Met5]脑啡肽、去甲硫氨酸-[Met5]脑啡肽和酪氨酰-甘氨酰-甘氨酸可增加自然杀伤细胞的细胞毒性以及丝裂原刺激的B细胞和T细胞的增殖。这些作用被纳洛酮预处理所抑制，这提示了所研究肽类的阿片样物质机制。淋巴细胞增殖率不仅在单次注射[Met5]脑啡肽或其代谢产物后增加，而且在治疗3天或7天后也增加。除了功能作用外，[Met5]脑啡肽、去甲硫氨酸-[Met5]脑啡肽和酪氨酰-甘氨酰-甘氨酸还增加了自然杀伤细胞和T细胞的百分比。本研究结果表明，[Met5]脑啡肽的免疫调节作用可能由其N端代谢产物去甲硫氨酸-[Met5]脑啡肽和酪氨酰-甘氨酰-甘氨酸介导或增强。
