Matsuda K, Matsuda S, Ito Y
Laboratory of Biophysical Chemistry, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
J Chromatogr A. 1998 May 29;808(1-2):95-104. doi: 10.1016/s0021-9673(98)00114-9.
This paper deals with optimization of a new seal-free compact toroidal coil centrifuge to achieve high resolution in analytical counter-current chromatography (CCC). Toroidal coil CCC (hydrostatic motion) has advantages compared with high-speed CCC (efficiently mixing solution with planetary motion) in the separation of protein or easily emulsified samples. A toroidal coil separation column of 0.4 mm I.D. PTFE tubing was accommodated around the periphery of the cylindrical centrifuge bowl. Using a two-phase solvent system composed of chloroform-acetic acid-0.1 M hydrochloric acid (2:2:1, v/v/v) and a set of dinitrophenyl-amino acids as test samples, a series of experiments was performed with parameters such as the column length, sample volume, flow-rate, elution mode of the mobile phase and rotation speed. The highest efficiency, over 10,000 theoretical plates, was achieved with a 100 m long coiled tube and an 11 ml total capacity at a flow-rate of 0.01 ml/min at 800 rpm.
本文探讨了一种新型无密封紧凑型环形线圈离心机的优化，以在分析逆流色谱法（CCC）中实现高分辨率。与高速CCC（通过行星运动有效混合溶液）相比，环形线圈CCC（流体静力运动）在蛋白质或易乳化样品的分离方面具有优势。内径为0.4毫米的聚四氟乙烯（PTFE）管制成的环形线圈分离柱围绕圆柱形离心杯的周边放置。使用由氯仿 - 乙酸 - 0.1 M盐酸（2:2:1，v/v/v）组成的两相溶剂系统和一组二硝基苯基氨基酸作为测试样品，对柱长、样品体积、流速、流动相洗脱模式和转速等参数进行了一系列实验。在800转/分钟的转速下，以0.01毫升/分钟的流速，使用100米长的盘管和11毫升的总容量时，实现了最高效率，超过10,000个理论塔板。
