Ito Yoichiro, Yu Henry
Bioseparation Technology Laboratory, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bldg. 10, Rm 8N230, 10 Center Drive, Bethesda, MD 20892-1762, USA.
J Liq Chromatogr Relat Technol. 2009;32(4):560-566. doi: 10.1080/10826070802671580.
Effective column space and stationary phase retention have been improved by changing the configuration of the helical column originally used for toroidal coil countercurrent chromatography. The use of an equilateral triangular core for the helix column doubles effective column space and retains the stationary phase over 40% of the total column capacity without increasing the column pressure. The present results suggest that the stationary phase retention and the peak resolution will be further improved using new column designs fabricated by a new technology called "laser sintering for rapid prototyping."
通过改变最初用于环形盘管逆流色谱的螺旋柱的结构，有效柱空间和固定相保留率得到了提高。使用等边三角形芯的螺旋柱使有效柱空间增加了一倍，并在不增加柱压的情况下，将固定相保留率保持在总柱容量的40%以上。目前的结果表明，使用一种名为“激光烧结快速成型”的新技术制造的新型柱设计，固定相保留率和峰分辨率将得到进一步提高。
