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高效液相色谱法测定各类食品及配方中L-天冬氨酰-L-苯丙氨酸甲酯的含量。

High-performance liquid chromatographic determination of L-aspartyl-L-phenylalanine methyl ester in various food products and formulations.

作者信息

Fox L, Anthony G D, Lau E P

出版信息

J Assoc Off Anal Chem. 1976 Sep;59(5):1048-50.

PMID:965327
Abstract

A simple, rapid, and specific high-performance liquid chromatographic (HPLC) procedure is described for the analysis of the chemical sweetener L-aspartyl-L-phenylalanine methyl ester (aspartame). Using a strong cation exchange column and pressures less than 1000 psig, an analysis can be performed in less than 15 min. The technique has been applied to a wide range of food products and formulations. No interferences were found in the samples studied. Recoveries are quantitative, and the coefficients of variation for replicate analyses are less than or equal to 2.5%.

摘要

本文描述了一种用于分析化学甜味剂L-天冬氨酰-L-苯丙氨酸甲酯(阿斯巴甜)的简单、快速且特异的高效液相色谱(HPLC)方法。使用强阳离子交换柱且压力低于1000 psig,分析可在不到15分钟内完成。该技术已应用于多种食品和配方。在所研究的样品中未发现干扰。回收率为定量,重复分析的变异系数小于或等于2.5%。

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