Eamsobhana P, Mak J W, Yong H S
Department of Medical Sciences, Ministry of Public Health, Bangkok, Thailand.
Southeast Asian J Trop Med Public Health. 1997;28 Suppl 1:139-42.
A specific monoclonal antibody (AW-3C2) as revealed by ELISA was produced against the adult worm antigens of Parastrongylus cantonensis and used in a sandwich ELISA for the detection of circulating antigens in the sera of parastrongyliasis patients and those with other parasitic diseases. A total of 60 sera was used in this study. Of these, 10 each were from patients with parastrongyliasis filariasis, gnathostomiasis, malaria and toxocariasis. The control group consisted of 53 serum samples from normal healthy Thais and Malaysians. The mean +/- optical density (OD) values for the normal Thai and Malaysian groups were 0.126 +/- 0.028 and 0.124 +/- 0.029, respectively. Mean OD values of parastrongyliasis patient group differed significantly from that of the normal groups as well as those of other parasitic infections. Using a cut-off point of mean OD +/- 3SD of the normal control groups as indicating a positive reading, the specificity of the assay with this monoclonal antibody was 100% while the sensitivity was 50%.
通过酶联免疫吸附测定（ELISA）发现，一种针对广州管圆线虫成虫抗原产生的特异性单克隆抗体（AW-3C2）被用于夹心ELISA，以检测广州管圆线虫病患者和其他寄生虫病患者血清中的循环抗原。本研究共使用了60份血清。其中，分别有10份来自广州管圆线虫病、丝虫病、颚口线虫病、疟疾和弓蛔虫病患者。对照组由53份来自正常健康泰国人和马来西亚人的血清样本组成。泰国和马来西亚正常组的平均±光密度（OD）值分别为0.126±0.028和0.124±0.029。广州管圆线虫病患者组的平均OD值与正常组以及其他寄生虫感染组的平均OD值有显著差异。以正常对照组平均OD±3标准差作为阳性读数的截断点，该单克隆抗体检测方法的特异性为100%，而敏感性为50%。
