使用基于单克隆抗体的酶联免疫吸附测定法（MAb-ELISA）检测马来西亚管圆线虫循环抗原。

Detection of Angiostrongylus malaysiensis circulating antigen using monoclonal antibody-based enzyme-linked immunosorbent assay (MAb-ELISA).

作者信息

Ambu S, Rain A N, Mak J W, Maslah D, Maidah S

机构信息

Institute for Medical Research, Jalan Pahang, Kuala Lumpur, Malaysia.

出版信息

Southeast Asian J Trop Med Public Health. 1997;28 Suppl 1:143-7.

PMID:9656366

Abstract

Three MAbs 1C4.2D8, 1C4.2C4 and 1C4.1F5 were produced using sonicated adult worm antigens of Angiostrongylus malaysiensis and they were found to be secreters of IgG1. The MAbs 1C4.2C4 and 1C4.2D8 were found to react with antigens of A. malaysiensis and cross-react with the closely related A. cantonensis but not with other helminths. A total of 108 human sera collected from Orang Asli (aborigenes) from Grik, in the State of Perak were tested for A. malaysiensis infection using the MAb-ELISA. MAb 1C4.1F5 and 25 (23%) were positive. Twenty of these positive samples were tested with the MAb 1C4.2D8 and none was found to be positive.

摘要

使用马来西亚管圆线虫成虫经超声处理后的抗原制备了三种单克隆抗体1C4.2D8、1C4.2C4和1C4.1F5，发现它们是IgG1的分泌型抗体。发现单克隆抗体1C4.2C4和1C4.2D8与马来西亚管圆线虫抗原发生反应，并与密切相关的广州管圆线虫发生交叉反应，但不与其他蠕虫发生反应。使用单克隆抗体酶联免疫吸附测定法对从霹雳州格里克的奥朗阿斯利人（原住民）收集的总共108份人类血清进行了马来西亚管圆线虫感染检测。单克隆抗体1C4.1F5检测结果显示25份（23%）呈阳性。对其中20份阳性样本用单克隆抗体1C4.2D8进行检测，未发现阳性样本。